PDF(Pubmed)
Abstract：
The study aimed to investigate molecularly the presence of flea-borne viruses in infested small ruminants with fleas. It was carried out in Egypt\'s Northern West Coast (NWC) and South Sinai Governorate (SSG). Three specific primers were used targeting genes, ORF103 (for Capripoxvirus and Lumpy skin disease virus), NS3 (for Bluetongue virus), and Rdrp (for Coronavirus), followed by gene sequencing and phylogenetic analyses. The results revealed that 78.94% of sheep and 65.63% of goats were infested in the NWC area, whereas 49.76% of sheep and 77.8% of goats were infested in the SSG region. Sheep were preferable hosts for flea infestations (58.9%) to goats (41.1%) in the two studied areas. Sex and age of the animals had no effects on the infestation rate (p > 0.05). The season and site of infestation on animals were significantly different between the two areas (p < 0.05). Ctenocephalides felis predominated in NWC and Ctenocephalides canis in SSG, and males of both flea species were more prevalent than females. Molecular analysis of flea DNA revealed the presence of Capripoxvirus in all tested samples, while other viral infections were absent. Gene sequencing identified three isolates as sheeppox viruses, and one as goatpox virus. The findings suggest that Capripoxvirus is adapted to fleas and may be transmitted to animals through infestation. This underscores the need for ongoing surveillance of other pathogens in different regions of Egypt.
摘要：
该研究旨在从分子上研究跳蚤传播病毒在有跳蚤感染的小反刍动物中的存在。它是在埃及的西北海岸（NWC）和南西奈省（SSG）进行的。使用三种特异性引物靶向基因，ORF103（用于羊痘病毒和块状皮肤病病毒），NS3（用于蓝舌病毒），和Rdrp（冠状病毒），其次是基因测序和系统发育分析。结果表明,NWC地区有78.94%的绵羊和65.63%的山羊出没,而在SSG地区有49.76％的绵羊和77.8％的山羊被感染。在两个研究区域中，绵羊是跳蚤侵染的首选宿主（58.9％），而不是山羊（41.1％）。动物的性别和年龄对侵染率没有影响(p>0.05)。动物侵染的季节和部位在两个区域之间显著不同(p<0.05)。Ctenocephalidesfelis在NWC中占主导地位，在SSG中Ctenocephalides犬占主导地位，两种跳蚤的雄性比雌性更普遍。跳蚤DNA的分子分析显示所有测试样品中都存在Capropoxvirus，而没有其他病毒感染。基因测序确定了三个分离株为羊痘病毒，还有一种是山羊痘病毒.研究结果表明，Capripoxvirus适用于跳蚤，并可能通过感染传播给动物。这突出表明需要在埃及不同地区持续监测其他病原体。
