PDF(Pubmed)
Abstract：
Biological agents are getting a noticeable concern as efficient eco-friendly method for nanoparticle fabrication, from which fungi considered promising agents in this field. In the current study, two fungal species (Embellisia spp. and Gymnoascus spp.) were isolated from the desert soil in Saudi Arabia and identified using 18S rRNA gene sequencing then used as bio-mediator for the fabrication of silver nanoparticles (AgNPs). Myco-synthesized AgNPs were characterized using UV-visible spectrometry, transmission electron microscopy, Fourier transform infrared spectroscopy and dynamic light scattering techniques. Their antibacterial activity against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Klebsiella pneumoniae were investigated. In atrial to detect their possible antibacterial mechanism, Sodium dodecyl sulfate (SDS-PAGE) and TEM analysis were performed for Klebsiella pneumoniae treated by the myco-synthesized AgNPs. Detected properties of the fabricated materials indicated the ability of both tested fungal strains in successful fabrication of AgNPs having same range of mean size diameters and varied PDI. The efficiency of Embellisia spp. in providing AgNPs with higher antibacterial activity compared to Gymnoascus spp. was reported however, both indicated antibacterial efficacy. Variations in the protein profile of K. pneumoniae after treatments and ultrastructural changes were observed. Current outcomes suggested applying of fungi as direct, simple and sustainable approach in providing efficient AgNPs.
摘要：
生物制剂作为纳米粒子制造的有效生态友好方法正受到关注，真菌认为该领域有前途的药物。在目前的研究中,两种真菌（Embellisiaspp。和金曲霉属。）从沙特阿拉伯的沙漠土壤中分离出来，并使用18SrRNA基因测序进行鉴定，然后用作制造银纳米颗粒（AgNP）的生物介质。Myco合成的AgNPs使用紫外-可见光谱法进行表征，透射电子显微镜,傅里叶变换红外光谱和动态光散射技术。它们对大肠杆菌的抗菌活性，铜绿假单胞菌,金黄色葡萄球菌,并对肺炎克雷伯菌进行了调查。在心房中检测它们可能的抗菌机制，对通过myco合成的AgNP处理的肺炎克雷伯菌进行十二烷基硫酸钠(SDS-PAGE)和TEM分析。所制造材料的检测特性表明两种测试真菌菌株成功制造具有相同范围的平均尺寸直径和不同PDI的AgNP的能力。Embellisiaspp的效率。与红曲霉属相比，AgNPs具有更高的抗菌活性。然而，据报道，两者都表明抗菌功效。观察到治疗后肺炎克雷伯菌蛋白质谱的变化和超微结构变化。目前的结果表明，直接应用真菌，提供有效的AgNPs的简单和可持续的方法。
