Abstract：
The poliovirus (PV) enters the central nervous system (CNS) via the bloodstream, suggesting the existence of a mechanism to cross the blood-brain barrier. Here, we report that PV capsid proteins (VP1 and VP3) can penetrate cells, with VP3 being more invasive. Two independent parts of VP3 are responsible for this function. Both peptides can penetrate human umbilical cord vascular endothelial cells, and one peptide of VP3 could also penetrate peripheral blood mononuclear cells. In an in vitro blood-brain barrier model using rat-derived astrocytes, pericytes, and endothelial cells, both peptides were observed to traverse from the blood side to the brain side at 6 h after administration. These results provide insights into the molecular mechanisms underlying PV invasion into the CNS.
摘要：
脊髓灰质炎病毒（PV）通过血液进入中枢神经系统（CNS），表明存在一种穿过血脑屏障的机制。这里,我们报道PV衣壳蛋白（VP1和VP3）可以穿透细胞，VP3更具侵入性。VP3的两个独立部分负责此功能。两种肽都能穿透人脐带血管内皮细胞，VP3的一种肽也可以穿透外周血单核细胞。在使用大鼠来源的星形胶质细胞的体外血脑屏障模型中，周细胞,和内皮细胞，在给药后6小时，观察到两种肽都从血液侧穿行到大脑侧。这些结果提供了对PV侵入CNS的潜在分子机制的见解。
