Abstract：
pH could play vital role in the wound healing process due to the bacterial metabolites, which is one essential aspect of desirable wound dressings lies in being pH-responsive. This work has prepared a degradable hyaluronic acid hydrogel dressing with wound pH response-ability. The aldehyde-modified hyaluronic acid (AHA) was obtained, followed by complex mixture formation of eugenol and oregano antibacterial essential oil in the AHA-CMCS hydrogel through the Schiff base reaction with carboxymethyl chitosan (CMCS). This hydrogel composite presents pH-responsiveness, its disintegration mass in acidic environment (pH = 5.5) is 4 times that of neutral (pH = 7.2), in which the eugenol release rate increases from 37.6 % to 82.1 %. In vitro antibacterial and in vivo wound healing investigations verified that hydrogels loaded with essential oils have additional 5 times biofilm removal efficiency, and significantly accelerate wound healing. Given its excellent anti-biofilm and target-release properties, the broad application of this hydrogel in bacteria-associated wound management is anticipated.
摘要：
由于细菌代谢产物的存在,pH值在伤口愈合过程中起着至关重要的作用,理想的伤口敷料的一个基本方面在于具有pH响应性。这项工作已经制备了具有伤口pH响应能力的可降解透明质酸水凝胶敷料。获得醛改性透明质酸(AHA),然后通过席夫碱与羧甲基壳聚糖（CMCS）反应，在AHA-CMCS水凝胶中形成丁香酚和牛至抗菌精油的复杂混合物。这种水凝胶复合材料具有pH响应性，其在酸性环境（pH=5.5）中的崩解量是中性环境（pH=7.2）的4倍，其中丁香酚释放率从37.6%提高到82.1%。体外抗菌和体内伤口愈合研究证实，负载精油的水凝胶具有额外的5倍生物膜去除效率，并显著加速伤口愈合。鉴于其优异的抗生物膜和靶标释放特性，预计该水凝胶在细菌相关伤口处理中的广泛应用。
