Abstract：
Hydrogels based on poly(vinyl alcohol), silk sericin, and gelatin containing Camellia oleifera oil (CO)-loaded chitosan nanoparticles (CSNPs) were fabricated. The loading of CO into CSNPs was achieved by a two-step procedure, which included an oil-in-water emulsion and an ionic gelation method. SEM images of CO-loaded CSNPs illustrated the spherical shape with aggregation of the nanoparticles. The particle size and polydispersity index were 541-1089 nm and 0.39-0.65, respectively. The encapsulation efficiency and loading capacity were 3-16 % and 4-6 %, respectively. The gelatin/poly(vinyl alcohol)/sericin hydrogels were fabricated and incorporated with CO or CO-loaded CSNPs with different concentrations of CO-loaded CSNPs. All hydrogels demonstrated a porous structure. Besides, the hydrogels containing CO-loaded CSNPs showed a more controlled and sustained release profile than the hydrogels containing CO. Moreover, the hydrogels showed tyrosinase inhibition (9-13 %) and antioxidant activity (37-60 %). Finally, the hydrogels containing CO-loaded CSNPs were non-toxic to the Normal Human Dermal Fibroblasts and NCTC clone 929 cells, even at a high dosage of 50 mg/mL. As a result, these hydrogels exhibited excellent potential for use in cosmeceutical industries.
摘要：
基于聚乙烯醇的水凝胶，丝胶,制备了含有油茶油（CO）的明胶的壳聚糖纳米颗粒（CSNPs）。通过两步程序将CO加载到CSNP中，其中包括水包油乳液和离子凝胶法。CO负载的CSNP的SEM图像示出了具有纳米颗粒聚集的球形形状。粒径和多分散指数分别为541-1089nm和0.39-0.65。封装效率和负载能力分别为3-16%和4-6%，分别。制备明胶/聚(乙烯醇)/丝胶凝胶,并掺入具有不同浓度的CO负载CSNP的CO或CO负载CSNP。所有水凝胶都表现出多孔结构。此外,含有CO负载的CSNP的水凝胶显示出比含有CO的水凝胶更受控和持续的释放曲线。此外，水凝胶显示酪氨酸酶抑制（9-13％）和抗氧化活性（37-60％）。最后,含有CO负载CSNP的水凝胶对正常人真皮成纤维细胞和NCTC克隆929细胞无毒，即使在50毫克/毫升的高剂量。因此,这些水凝胶在化妆品行业中表现出极好的应用潜力。
