Abstract：
Integrating pH sensor with controlled antibiotic release is fabricated on silk to create a theranostic wound dressing. Alginate (ALG) hydrogel and graphene oxide (GO) loaded with levofloxacin (LVX) and a pH indicator are applied to fabricate a pH-responsive theranostic wound dressing. The modified silk color changes from yellow to green in response to elevated skin pH, indicating the skin infection. The semi-quantitative analysis was conducted using ImageJ, revealing significant color changes across the wide range. At elevated pH levels, the ionization of the COOH bonds within ALG induces repulsion among the COO- groups, thereby accelerating the release of the incorporated drug compared to release under lower pH. At an infected pH of 8, ALG hydrogel triggers LVX releasing up to 135.86 ± 0.3 µg, while at a normal pH of 7, theranostic silk releases 123.13 ± 0.26 µg. Incorporating GO onto silk fibers enhances LVX loading and sustains LVX release. Furthermore, these modified silks possess antimicrobial abilities without causing irritation or allergies on the human skin. This theranostic silks represents a major step forward in smart wound care, introducing a versatile platform of smart wound care.
摘要：
在丝上制造具有受控抗生素释放的集成pH传感器，以创建治疗性伤口敷料。应用加载有左氧氟沙星(LVX)和pH指示剂的海藻酸盐(ALG)水凝胶和氧化石墨烯(GO)来制造pH响应性治疗伤口敷料。修改后的丝绸颜色从黄色变为绿色，以响应皮肤pH值的升高，提示皮肤感染。使用ImageJ进行半定量分析，揭示了广泛的颜色变化。在升高的pH水平，ALG中COOH键的电离会引起COO-基团之间的排斥，因此,与在较低pH下的释放相比,加速了掺入的药物的释放。在感染的pH值为8时，ALG水凝胶触发LVX释放高达135.86±0.3µg，而在正常pH值为7时，theranostic丝释放出123.13±0.26µg。将GO结合到丝纤维上增强LVX负载并维持LVX释放。此外，这些改性的丝绸具有抗菌能力，不会对人体皮肤造成刺激或过敏。这种治疗丝绸代表了智能伤口护理的重要一步，引入一个多功能的智能伤口护理平台。
