Abstract：
Accurate and sensitive determination of human immunoglobulin G (HIgG) level is critical for diagnosis and treatment of various diseases, including rheumatoid arthritis, humoral immunodeficiencies, and infectious disease. In this study, versatile tri-signal probes were developed by preparing CdS@SiO2 nanorods that integrate photoluminescence (PL), multi-phonon resonant Raman scattering (MRRS) and infrared absorption (IRA) properties. Through the coating of multiple CdS nanoparticles as cores within SiO2 shells, the PL and MRRS properties of CdS were improved, resulting in a significantly lowered limit of detection (LOD), with the lowest LOD of 12.37 ag mL-1. Integration with the distinctive IRA property of SiO2 shells widened the detection range towards higher concentrations, establishing a final linear range of 50 ag mL-1 to 10 μg mL-1. The remarkable consistency among the three signals highlighted the robust internal verification capability for accurate detection. This approach enhances flexibility in selecting detection methodologies to suit diverse scenarios, facilitating HIgG detection. The tri-signal nanoprobes also exhibited excellent detection selectivity, specificity and repeatability. This study presents a fresh idea for developing high-performance detection strategies.
摘要：
准确、灵敏地测定人免疫球蛋白G(HIgG)水平对各种疾病的诊断和治疗至关重要,包括类风湿性关节炎,体液免疫缺陷，和传染病。在这项研究中,通过制备整合光致发光（PL）的CdS@SiO2纳米棒，开发了通用的三信号探针，多声子共振拉曼散射(MRRS)和红外吸收(IRA)特性。通过包覆多个CdS纳米粒子作为SiO2壳内的核心，提高了CdS的PL和MRRS特性，导致检测限（LOD）显着降低，最低LOD为12.37agmL-1。与SiO2壳的独特IRA特性相结合，将检测范围扩大到更高的浓度，建立50agmL-1至10μgmL-1的最终线性范围。三个信号之间的显着一致性突出了准确检测的强大内部验证能力。这种方法增强了选择检测方法以适应不同场景的灵活性，促进HIgG检测。三信号纳米探针还表现出优异的检测选择性，特异性和可重复性。本研究为开发高性能检测策略提供了新的思路。
