Abstract：
As the third active gas signal molecule in plants, hydrogen sulfide (H2S) plays important roles in physiological metabolisms and biological process of fruits and vegetables during postharvest storage. In the present study, the effects of H2S on enhancing resistance against soft rot caused by Botryosphaeria dothidea and the involvement of jasmonic acid (JA) signaling pathway in kiwifruit during the storage were investigated. The results showed that 20 μL L-1 H2S fumigation restrained the disease incidence of B. dothidea-inoculated kiwifruit during storage, and delayed the decrease of firmness and the increase of soluble solids (SSC) content. H2S treatment increased the transcription levels of genes related to JA biosynthesis (AcLOX3, AcAOS, AcAOC2, and AcOPR) and signaling pathway (AcCOI1, AcJAZ5, AcMYC2, and AcERF1), as well as the JA accumulation. Meanwhile, H2S promoted the expression of defense-related genes (AcPPO, AcSOD, AcGLU, AcCHI, AcAPX, and AcCAT). Correlation analysis revealed that JA content was positively correlated with the expression levels of JA biosynthesis and defense-related genes. Overall, the results indicated that H2S could promote the increase of endogenous JA content and expression of defense-related genes by regulating the transcription levels of JA pathway-related genes, which contributed to the inhibition on the soft rot occurrence of kiwifruit.
摘要：
作为植物中的第三个活性气体信号分子，硫化氢(H2S)在果蔬采后贮藏的生理代谢和生物过程中起着重要作用。在本研究中,研究了H2S对猕猴桃贮藏过程中抗软腐病的影响以及茉莉酸（JA）信号通路的参与。结果表明，20μL-1H2S熏蒸抑制了接种B.dophidea的猕猴桃在贮藏期间的发病率。并延缓了硬度的降低和可溶性固形物（SSC）含量的增加。H2S处理增加了与JA生物合成相关的基因的转录水平(AcLOX3，AcAOS，AcAOC2和AcOPR）和信号通路（AcCOI1，AcJAZ5，AcMYC2和AcERF1），以及JA的积累。同时,H2S促进防御相关基因的表达(AcPPO，AcSOD,AcGLU,Acchi,AcAPX,和AcCAT）。相关分析表明，JA含量与JA生物合成和防御相关基因的表达水平呈正相关。总的来说,结果表明,H2S可通过调节JA通路相关基因的转录水平,促进内源性JA含量的增加和防御相关基因的表达,这有助于抑制猕猴桃软腐病的发生。
