Abstract：
Epstein-Barr virus (EBV) is a ubiquitous and oncogenic virus that is associated with various malignancies and non-malignant diseases and EBV DNA detection is widely used for the diagnosis and prognosis prediction for these diseases. The dried blood spots (DBS) sampling method holds great potential as an alternative to venous blood samples in geographically remote areas, for individuals with disabilities, or for newborn blood collection. Therefore, the objective of this study was to assess the viability of detecting EBV DNA load from DBS. Matched whole blood and DBS samples were collected for EBV DNA extraction and quantification detection. EBV DNA detection in DBS presented a specificity of 100 %. At different EBV DNA viral load in whole blood, the sensitivity of EBV DNA detection in DBS was 38.78 % (≥1 copies/mL), 43.18 % (≥500 copies/mL), 58.63 % (≥1000 copies/mL), 71.43 % (≥2000 copies/mL), 82.35 % (≥4000 copies/mL), and 92.86 % (≥5000 copies/mL), respectively. These results indicated that the sensitivity of EBV DNA detection in DBS increased with elevating viral load. Moreover, there was good correlation between EBV DNA levels measured in whole blood and DBS, and on average, the viral load measured in whole blood was about 6-fold higher than in DBS. Our research firstly demonstrated the feasibility of using DBS for qualitative and semi-quantitative detection of EBV DNA for diagnosis and surveillance of EBV-related diseases.
摘要：
EB病毒（EBV）是一种广泛存在的致癌病毒，与多种恶性肿瘤和非恶性肿瘤相关，EBVDNA检测被广泛用于这些疾病的诊断和预后预测。干血点（DBS）采样方法具有巨大的潜力，可以替代偏远地区的静脉血样本。对于残疾人来说，或新生儿采血。因此,这项研究的目的是评估从DBS检测EBVDNA负载的可行性。收集匹配的全血和DBS样品用于EBVDNA提取和定量检测。在DBS中的EBVDNA检测呈现100%的特异性。在全血中不同的EBVDNA病毒载量下，在DBS中检测EBVDNA的灵敏度为38.78％（≥1拷贝/mL），43.18%（≥500拷贝/mL），58.63%（≥1000拷贝/mL），71.43%（≥2000拷贝/mL），82.35%（≥4000拷贝/mL），和92.86%（≥5000拷贝/毫升），分别。这些结果表明，随着病毒载量的增加，DBS中EBVDNA检测的灵敏度增加。此外，在全血和DBS中测量的EBVDNA水平之间存在良好的相关性，平均而言,在全血中测得的病毒载量比DBS高约6倍.我们的研究首次证明了使用DBS定性和半定量检测EBVDNA用于诊断和监测EBV相关疾病的可行性。
