Abstract：
Agricultural production relies heavily on the use of pesticides, which may accumulate in soil and water, posing a significant threat to the global ecological environment and biological health. Butachlor is a commonly used herbicide and environmental pollutant, which has been linked to liver and kidney damage, as well as neurological abnormalities. However, the potential impact of butachlor exposure on the gut microbiota remains understudied. Thus, our aim was to investigate the potential negative effects of butachlor exposure on host health and gut microbiota. Our results demonstrated that butachlor exposure significantly reduced the host antioxidant capacity, as evidenced by decreased levels of T-AOC, SOD, and GSH-Px, and increased levels of MDA. Serum biochemical analysis also revealed a significant increase in AST and ALT levels during butachlor exposure. Microbial analysis showed that butachlor exposure significantly reduced the abundance and diversity of gut microbiota. Furthermore, butachlor exposure also significantly altered the gut microbial composition. In conclusion, our findings indicate that butachlor exposure can have detrimental health effects, including dysregulation of antioxidant enzymes, abnormalities in transaminases, and hepatointestinal damage. Furthermore, it disrupts the gut microbial homeostasis by altering microbial composition and reducing diversity and abundance. In the context of the increasingly serious use of pesticides, this study will help provide impetus for standardizing the application of pesticides and reducing environmental pollution.
摘要：
农业生产严重依赖农药的使用,它们可能会积聚在土壤和水中，对全球生态环境和生物健康构成重大威胁。丁草胺是一种常用的除草剂和环境污染物,这与肝脏和肾脏损伤有关，以及神经系统异常。然而,丁草胺暴露对肠道微生物群的潜在影响仍未得到充分研究。因此，我们的目的是研究丁草胺暴露对宿主健康和肠道微生物群的潜在负面影响。我们的结果表明，丁草胺暴露显著降低了宿主的抗氧化能力，T-AOC水平下降证明，SOD,和GSH-Px,和MDA水平增加。血清生化分析还显示，丁草胺暴露期间AST和ALT水平显着增加。微生物分析表明丁草胺暴露显著降低了肠道菌群的丰度和多样性。此外，丁草胺暴露也显着改变了肠道微生物组成。总之,我们的研究结果表明，接触丁草胺会对健康产生有害影响，包括抗氧化酶的失调，转氨酶异常，和肝肠损伤.此外，它通过改变微生物组成和减少多样性和丰度来破坏肠道微生物稳态。在农药使用日益严重的背景下,这项研究将有助于为规范农药的使用和减少环境污染提供动力。
