Abstract：
Constructing metagenome-assembled genomes (MAGs) from complex metagenomic samples involves a series of bioinformatics operations, each requiring deep bioinformatics knowledge. Here, we present a protocol for constructing MAGs and conducting functional profiling to address biological questions. We describe steps for system configuration, data downloads, read processing, removal of human DNA contamination, metagenomic assembly, and statistical quality assessment of the final assembly. Additionally, we detail procedures for the construction and refinement of MAGs, as well as the functional profiling of MAGs.
摘要：
从复杂的宏基因组样本构建宏基因组组装基因组（MAG）涉及一系列生物信息学操作，每个人都需要深厚的生物信息学知识。这里,我们提出了构建MAG并进行功能分析以解决生物学问题的方案.我们描述了系统配置的步骤，数据下载,读取处理,去除人类DNA污染，宏基因组组装，和最终装配的统计质量评估。此外,我们详细介绍了MAG的构建和细化程序，以及MAG的功能分析。
