Abstract：
Mitochondrial genome is an important molecular marker for exploring the phylogenetic relationships of species and revealing molecular evolution. In the present study, 5 mitogenomes of Chromodorididae (Chromodoris lochi, Chromodoris colemani, Chromodoris elisabethina, Chromodoris annae and Hypselodoris whitei) were systemically investigated. The lengths of the mitogenomes sequences were 14248 bp, 14257 bp, 14252 bp, 14254 bp and 14856 bp, respectively. Most protein-coding genes (PCGs) were initiated with the common ATG codon and terminated with the TAA and TAG. We calculated Ka/Ks values for all 13 PCGs of Chromodorididae species, all ratios were less than 1, indicating selection by purification. Phylogenetic relationships were constructed by Bayesian inference (BI) and maximum likelihood (ML) methods based on all complete genomes of 50 species, primarily from the family Chromodorididae (Doridina) and 2 outgroups. This phylogenetic tree provided further additional references for the classification of the suborder Doridina. Gene rearrangement suggested a more conserved pattern of gene sequences in the superfamily Chromodoridoidea. These results and newly sequenced will contribute to a better understanding of Chromodorididae and provide reference for further phylogenetic studies.
摘要：
线粒体基因组是探索物种系统发育关系和揭示分子进化的重要分子标记。在本研究中,5个有丝分裂科（Chromodorislochi，Chromodoriscolemani,Chromodoriselisabethina,对银杏和白药）进行了系统研究。有丝分裂基因组序列的长度为14248bp，14257bp,14252bp，14254bp和14856bp，分别。大多数蛋白质编码基因(PCGs)用共同的ATG密码子起始,并用TAA和TAG终止。我们计算了所有13种色源科PCG的Ka/Ks值，所有比率均小于1，表明通过纯化进行选择。基于50个物种的所有完整基因组，通过贝叶斯推断（BI）和最大似然（ML）方法构建了系统发生关系，主要来自色科科（Doridina）和2个外群。该系统发育树为Doridina亚目的分类提供了进一步的参考。基因重排表明超家族Chromodoridoidea中基因序列的保守模式。这些结果和新的测序结果将有助于更好地了解彩色科，并为进一步的系统发育研究提供参考。
