Abstract：
Two Gram-stain-negative, facultative anaerobic, rod-shaped, motile bacterial strains, designated F26243T and F60267T were isolated from coastal sediment in Weihai, China. Strains F26243T and F60267T were grown at 4-40 °C (optimum 33 °C), pH 7.0-9.5 and pH 6.5-9.5 (optimum at pH 7.0), in the presence of 1.0-7.0% (w/v) NaCl (optimum 2.5%) and 1.0-12.0% (w/v) NaCl (optimum 2.0%), respectively. The 16S rRNA gene sequences phylogenetic analysis showed that strains F26243T and F60267T are closely related to the genus Marinobacter and exhibited the highest sequence similarities to Marinobacter salexigens HJR7T (97.7% and 98.0%, respectively), the similarity between two isolates was 96.7%. Strains F26243T and F60267T displayed genomic DNA G + C content of 53.6% and 53.8%, respectively. When compared to the M. salexigens HJR7T, the average nucleotide identity (ANI) values were 83.7% and 84.1%, and the percentage of conserved proteins (POCP) values were 79.9% and 84.6%, respectively. Ubiquinone 9 (Q-9) was the only respiratory quinone detected in both isolates. The major cellular fatty acids (> 10.0%) were summed feature 3 (comprising C16:1ω7c and/or C16:1ω6c), C16:0 and C18:1ω9c. The polar lipid profiles of strains F26243T and F60267T contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidyldimethylethanolamine, phosphatidylglycerol, aminophospholipid and one unidentified phospholipid. Based on genomic characteristics, phenotypic and chemotaxonomic, strains F26243T and F60267T represent two novel species of the genus Marinobacter, for which the names Marinobacter sediminicola sp. nov. and Marinobacter xiaoshiensis sp. nov. are proposed, the type strains are F26243T (= KCTC 92640T = MCCC 1H01345T) and F60267T (= KCTC 92638T = MCCC 1H01346T).
摘要：
两个革兰氏染色阴性，兼性厌氧,杆状,活动细菌菌株，从威海沿海沉积物中分离出F26243T和F60267T，中国。菌株F26243T和F60267T在4-40°C（最佳33°C）下生长，pH7.0-9.5和pH6.5-9.5（最佳pH7.0），在1.0-7.0％（w/v）NaCl（最佳2.5％）和1.0-12.0％（w/v）NaCl（最佳2.0％）的存在下，分别。16SrRNA基因序列系统发育分析表明，菌株F26243T和F60267T与马氏杆菌属密切相关，并表现出最高的序列相似性（97.7%和98.0%，分别)，两个分离株的相似性为96.7%。菌株F26243T和F60267T基因组DNAG+C含量分别为53.6%和53.8%,分别。当与M.salexigensHJR7T相比时，平均核苷酸同一性（ANI）值分别为83.7%和84.1%，保守蛋白（POCP）值的百分比分别为79.9％和84.6％，分别。泛醌9（Q-9）是两种分离物中唯一检测到的呼吸醌。主要的细胞脂肪酸（>10.0％）的总和特征3（包括C16：1ω7c和/或C16：1ω6c），C16:0和C18:1ω9c。菌株F26243T和F60267T的极性脂质谱含有二磷脂酰甘油，磷脂酰乙醇胺，磷脂酰二甲基乙醇胺，磷脂酰甘油，氨基磷脂和一种不明磷脂。基于基因组特征，表型和化学分类学，菌株F26243T和F60267T代表了马氏杆菌属的两个新物种，为此，命名为金黄色葡萄球菌。11月。和咸鱼杆菌。11月。被提议，菌株类型为F26243T（=KCTC92640T=MCCC1H01345T）和F60267T（=KCTC92638T=MCCC1H01346T）。
