Abstract：
Two Gram-staining-negative, facultative anaerobic, rod-shaped and phosphate-solubilizing strains designated SG2303T and SG2305, were isolated from paddy soil in China. Phylogenetic analysis based on 16 S rRNA gene sequences indicated that SG2303T and SG2305 represented a member of the genus Crenobacter within the family Neisseriaceae of the phylum Pseudomonadota. Strain SG2303T displayed higher 16 S rRNA gene sequence similarities with members of the genus Crenobacter ranging from 93.5 to 94.0%. Strains C. luteus YIM 78141T and C. cavernae K1W11S-77T were closest related to the isolated strains and were considered as type strains. Growth of strain SG2303T occurred at 10-55 °C (optimum 37 °C), pH 5.0-9.0 (optimum pH 6.0-7.0) and 0-1% (w/v) NaCl (optimum 0%). The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain SG2303T and its closely related taxa were 76.1-78.2% and 20.5-22.1%, respectively. The genomic DNA G + C content was 62.2%. The quinone of strain SG2303T was Q-8. The major fatty acids (> 10%) of strain SG2303T were C16:0 (30.6%), summed feature 3 (C16:1ω7c and/or C16:1ω6c) (26.0%) and C12:0 3OH (12.1%). The polar lipids were phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phospholipids (PL), glycolipid (GL) and unidentified lipids (UL). Based on the results of the phylogenetic, physiological, biochemical, and morphological analysis, strain SG2303T is recognized as a novel species of the genus Crenobacter, for which the name Crenobacter oryzisoli sp. nov. is proposed. The type strain is SG2303T (= GDMCC 1.3970T = JCM 36468T). In addition, SG2303T was also able of phosphorus solubilization and promoting the growth of rice seeds. Strain SG2303T exhibited a relatively high dissolvable phosphorus content of 2.52 µg·mL- 1.
摘要：
两种革兰氏染色阴性，兼性厌氧,从中国的稻田土壤中分离出杆状和溶解磷酸盐的菌株，命名为SG2303T和SG2305。基于16SrRNA基因序列的系统发育分析表明，SG2303T和SG2305代表假单胞菌门的奈瑟菌科内的Crenobacter属成员。菌株SG2303T与Crenobacter属的成员显示出较高的16SrRNA基因序列相似性，范围为93.5％至94.0％。黄体菌株YIM78141T和海绵状芽孢杆菌K1W11S-77T与分离的菌株关系最密切，被认为是类型菌株。菌株SG2303T的生长发生在10-55°C（最佳37°C），pH5.0-9.0（最佳pH6.0-7.0）和0-1％（w/v）NaCl（最佳0％）。菌株SG2303T及其密切相关的分类群之间的平均核苷酸同一性（ANI）和数字DNA-DNA杂交（dDDH）值分别为76.1-78.2％和20.5-22.1％，分别。基因组DNAG+C含量为62.2%。菌株SG2303T的醌为Q-8。菌株SG2303T的主要脂肪酸(>10%)为C16:0(30.6%),总计特征3(C16:1ω7c和/或C16:1ω6c)(26.0%)和C12:03OH(12.1%)。极性脂质是磷脂酰甘油（PG），二磷脂酰甘油（DPG），磷脂酰乙醇胺(PE),磷脂（PL），糖脂(GL)和未鉴定的脂质(UL)。根据系统发育的结果，生理,生物化学，和形态学分析，菌株SG2303T被认为是一种新型的杆菌属，其名称为Crenobacteroryzisolisp。11月。是提议的。菌株类型为SG2303T(=GDMCC1.3970T=JCM36468T)。此外,SG2303T还能够溶解磷并促进水稻种子的生长。菌株SG2303T表现出相对较高的可溶性磷含量，为2.52µg·mL-1。
