Abstract：
Two novel strain pairs (HM61T/HM23 and S-34T/S-58) were isolated from soil and the faeces of Tibetan antelope (Pantholops hodgsonii) collected at the Qinghai-Tibet Plateau of PR China. All four new isolates were aerobic, non-motile, Gram-stain-positive, catalase-positive, oxidase-negative, and short rod-shaped bacteria. The results of phylogenetic analysis based on the full-length 16S rRNA genes and 283 core genomic genes indicated that the four strains were separated into two independent branches belonging to the genus Nocardioides. Strains HM61T and HM23 were most closely related to Nocardioides pelophilus THG T63T (98.58 and 98.65 % 16S rRNA gene sequence similarity). Strains S-34T and S-58 were most closely related to Nocardioides okcheonensis MMS20-HV4-12T (98.89 and 98.89 % 16S rRNA gene sequence similarity). The G+C contents of the genomic DNA of strains HM61T and S-34T were 70.6 and 72.5 mol%, respectively. Strains HM61T, S-34T and the type strains of closely related species in the analysis had average nucleotide identity values of 75.4-90.5 % as well as digital DNA-DNA hybridization values between 20.1 and 40.8 %, which clearly indicated that the four isolates represent two novel species within the genus Nocardioides. The chemotaxonomic characteristics of strains HM61T and S-34T were consistent with the genus Nocardioides. The major fatty acids of all four strains were iso-C16 : 0, C17 : 1 ω8c or C18 : 1 ω9c. For strains HM61T and S-34T, MK-8(H4) was the predominant respiratory quinone, ll-2,6-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan, and the polar lipids profiles were composed of diphosphatidylglycerol and phosphatidylglycerol. Based on phylogenetic, phenotypic, and chemotaxonomic data, we propose that strains HM61T and S-34T represent two novel species of the genus Nocardioides, respectively, with the names Nocardioides bizhenqiangii sp. nov. and Nocardioides renjunii sp. nov. The type strains are HM61T (=GDMCC 4.343T=JCM 36399T) and S-34T (=CGMCC 4.7664T=JCM 33792T).
摘要：
从中国青藏高原收集的土壤和藏羚羊（Pantholopshodgsonii）的粪便中分离出两个新的菌株对（HM61T/HM23和S-34T/S-58）。所有四个新的分离株都是有氧的，不活动，革兰氏染色阳性,过氧化氢酶阳性,氧化酶阴性，和短杆状细菌。基于全长16SrRNA基因和283个核心基因组基因的系统发育分析结果表明，这四个菌株分为两个独立的分支，属于诺卡氏菌属。菌株HM61T和HM23与沙棘T63T最密切相关(98.58和98.65%的16SrRNA基因序列相似性)。菌株S-34T和S-58与大鸡诺氏MMS20-HV4-12T最密切相关（98.89和98.89％16SrRNA基因序列相似性）。HM61T和S-34T菌株基因组DNA的G+C含量分别为70.6和72.5mol%,分别。菌株HM61T,S-34T和分析中密切相关物种的类型菌株的平均核苷酸同一性值为75.4-90.5％，数字DNA-DNA杂交值在20.1和40.8％之间，这清楚地表明，这四个分离株代表了诺卡氏菌属中的两个新物种。菌株HM61T和S-34T的化学分类学特征与诺卡氏菌属一致。所有四个菌株的主要脂肪酸是异C16：0，C17：1ω8c或C18：1ω9c。对于菌株HM61T和S-34T，MK-8（H4）是主要的呼吸醌，11-2,6-二氨基庚二酸是细胞壁肽聚糖中的诊断二氨基酸，极性脂质谱由二磷脂酰甘油和磷脂酰甘油组成。基于系统发育，表型，和化学分类学数据，我们认为菌株HM61T和S-34T代表了诺卡氏菌属的两个新物种，分别,名称为诺卡氏菌。11月。和诺卡尼科动物。11月。菌株类型为HM61T(=GDMCC4.343T=JCM36399T)和S-34T(=CGMCC4.7664T=JCM33792T)。
