Abstract：
Fe-Ca-SAPO-34/CS/PANI, a novel hybrid bio-composite scaffold with potential application in dental tissue engineering, was prepared by freeze drying technique. The scaffold was characterized using FT-IR and SEM methods. The effects of PANI on the physicochemical properties of the Fe-Ca-SAPO-34/CS scaffold were investigated, including changes in swelling ratio, mechanical behavior, density, porosity, biodegradation, and biomineralization. Compared to the Fe-Ca-SAPO-34/CS scaffold, adding PANI decreased the pore size, porosity, swelling ratio, and biodegradation, while increasing the mechanical strength and biomineralization. Cell viability, cytotoxicity, and adhesion of human dental pulp stem cells (hDPSCs) on the scaffolds were investigated by MTT assay and SEM. The Fe-Ca-SAPO-34/CS/PANI scaffold promoted hDPSC proliferation and osteogenic differentiation compared to the Fe-Ca-SAPO-34/CS scaffold. Alizarin red staining, alkaline phosphatase activity, and qRT-PCR results revealed that Fe-Ca-SAPO-34/CS/PANI triggered osteoblast/odontoblast differentiation in hDPSCs through the up-regulation of osteogenic marker genes BGLAP, RUNX2, and SPARC. The significance of this study lies in developing a novel scaffold that synergistically combines the beneficial properties of Fe-Ca-SAPO-34, chitosan, and PANI to create an optimized microenvironment for dental tissue regeneration. These findings highlight the potential of the Fe-Ca-SAPO-34/CS/PANI scaffold as a promising biomaterial for dental tissue engineering applications, paving the way for future research and clinical translation in regenerative dentistry.
摘要：
Fe-Ca-SAPO-34/CS/PANI,一种新型的混合生物复合支架，在牙齿组织工程中具有潜在的应用，采用冷冻干燥技术制备。使用FT-IR和SEM方法对支架进行表征。研究了PANI对Fe-Ca-SAPO-34/CS支架理化性质的影响,包括溶胀率的变化，力学行为，密度,孔隙度,生物降解，和生物矿化。与Fe-Ca-SAPO-34/CS支架相比，添加PANI减小了孔径，孔隙度,溶胀率，和生物降解，同时增加机械强度和生物矿化。细胞活力，细胞毒性，通过MTT法和SEM研究了人牙髓干细胞（hDPSCs）在支架上的粘附。与Fe-Ca-SAPO-34/CS支架相比,Fe-Ca-SAPO-34/CS/PANI支架促进hDPSC增殖和成骨分化。茜素红染色，碱性磷酸酶活性，qRT-PCR结果显示,Fe-Ca-SAPO-34/CS/PANI通过上调成骨标记基因BGLAP,RUNX2和SPARC。这项研究的意义在于开发一种新型的支架，该支架协同地结合了Fe-Ca-SAPO-34，壳聚糖，和PANI，为牙齿组织再生创造优化的微环境。这些发现突出了Fe-Ca-SAPO-34/CS/PANI支架作为牙科组织工程应用的有前途的生物材料的潜力。为再生牙科的未来研究和临床翻译铺平了道路。
