Abstract：
Osteoblasts and osteoclasts are two of the most important types of cells in bone repair, and their bone-forming and bone-resorbing activities influence the process of bone repair. In this study, we proposed a physicochemical bidirectional regulation strategy via ration by physically utilizing hydroxyapatite nanopatterning to recruit and induce MSCs osteogenic differentiation and by chemically inhibiting osteolysis activity through the loaded zoledronate. The nanorod-like hydroxyapatite coating was fabricated via a modified hydrothermal process while the zoledronic acid was loaded through the chelation within the calcium ions. The fabrication of a hydroxyapatite/zoledronic acid composite biomaterial. This biomaterial promotes bone tissue regeneration by physically utilizing hydroxyapatite nanopatterning to recruit and induce MSCs osteogenic differentiation and by chemically inhibiting osteolysis activity through the loaded zoledronate. The nanorod-like hydroxyapatite coating was fabricated via a modified hydrothermal process while the zoledronic acid was loaded through the chelation within the calcium ions. The in vitro results tested on MSCs and RAW 246.7 indicated that the hydroxyapatite enhanced cells\' physical sensing system, therefore enhancing the osteogenesis. At the same time the zoledronic acid inhibited osteolysis by downregulating the RANK-related genes. This research provides a promising strategy for enhancing bone regeneration and contributes to the field of orthopedic implants.
摘要：
成骨细胞和破骨细胞是骨修复中最重要的两种细胞类型，骨形成和骨吸收活性影响骨修复过程。在这项研究中,我们提出了一种物理化学双向调节策略，通过定量通过物理利用羟基磷灰石纳米图案化招募和诱导MSCs成骨分化，并通过负载的唑来膦酸钠化学抑制骨溶解活性。纳米棒状羟基磷灰石涂层是通过改进的水热法制备的，同时唑来膦酸通过钙离子内的螯合作用加载。羟基磷灰石/唑来膦酸复合生物材料的制备。该生物材料通过物理地利用羟基磷灰石纳米图案化来募集和诱导MSC成骨分化以及通过负载的唑来膦酸盐化学抑制骨溶解活性来促进骨组织再生。纳米棒状羟基磷灰石涂层是通过改进的水热法制备的，同时唑来膦酸通过钙离子内的螯合作用加载。在MSCs和RAW246.7上测试的体外结果表明，羟基磷灰石增强了细胞的物理传感系统，因此增强成骨。同时唑来膦酸通过下调RANK相关基因抑制骨溶解。这项研究为增强骨骼再生提供了有希望的策略，并为骨科植入物领域做出了贡献。
