Abstract：
Repairing multiphasic defects is cumbersome. This study presents new soft and hard scaffold designs aimed at facilitating the regeneration of multiphasic defects by enhancing angiogenesis and improving cell attachment. Here, the nonimmunogenic, nontoxic, and cost-effective human serum albumin (HSA) fibril (HSA-F) was used to fabricate thermostable (up to 90 °C) and hard printable polymers. Additionally, using a 10.0 mg/mL HSA-F, an innovative hydrogel was synthesized in a mixture with 2.0% chitosan-conjugated arginine, which can gel in a cell-friendly and pH physiological environment (pH 7.4). The presence of HSA-F in both hard and soft scaffolds led to an increase in significant attachment of the scaffolds to the human periodontal ligament fibroblast (PDLF), human umbilical vein endothelial cell (HUVEC), and human osteoblast. Further studies showed that migration (up to 157%), proliferation (up to 400%), and metabolism (up to 210%) of these cells have also improved in the direction of tissue repair. By examining different in vitro and ex ovo experiments, we observed that the final multiphasic scaffold can increase blood vessel density in the process of per-vascularization as well as angiogenesis. By providing a coculture environment including PDLF and HUVEC, important cross-talk between these two cells prevails in the presence of roxadustat drug, a proangiogenic in this study. In vitro and ex ovo results demonstrated significant enhancements in the angiogenic response and cell attachment, indicating the effectiveness of the proposed design. This approach holds promise for the regeneration of complex tissue defects by providing a conducive environment for vascularization and cellular integration, thus promoting tissue healing.
摘要：
修复多相缺陷很麻烦。这项研究提出了新的软硬支架设计，旨在通过增强血管生成和改善细胞附着来促进多相缺陷的再生。这里,非免疫原性的，无毒,并且使用具有成本效益的人血清白蛋白（HSA）原纤维（HSA-F）来制造热稳定性（高达90°C）和硬质可印刷聚合物。此外,使用10.0mg/mL的HSA-F，一种创新的水凝胶是在与2.0％壳聚糖结合的精氨酸的混合物中合成的，可以在细胞友好和pH生理环境（pH7.4）中凝胶。HSA-F在硬支架和软支架中的存在导致支架与人牙周膜成纤维细胞（PDLF）的显着附着增加，人脐静脉内皮细胞（HUVEC），和人类成骨细胞。进一步的研究表明，迁移（高达157％），增殖（高达400%），并且这些细胞的代谢（高达210％）也在组织修复的方向上得到了改善。通过检查不同的体外和体外实验，我们观察到最终的多相支架可以增加血管形成和血管生成过程中的血管密度。通过提供包括PDLF和HUVEC在内的共培养环境，在罗沙司他药物的存在下，这两个细胞之间的重要串扰盛行，这项研究中的促血管生成。体外和体外结果表明血管生成反应和细胞附着的显着增强，表明拟议设计的有效性。这种方法通过为血管形成和细胞整合提供有利的环境，为复杂组织缺损的再生提供了希望。从而促进组织愈合。
