UNASSIGNED: Peritoneal lavage fluid from 50 patients with GC was obtained and processed via the isolation by size of epithelial tumor cells (ISET) method. Immunofluorescence and fluorescence in situ hybridization (FISH) were used to identify FCCs expressing chromosome 8 (CEP8), chromosome 17 (CEP17), and epithelial cell adhesion molecule (EpCAM).
UNASSIGNED: Using a combination of the ISET platform and immunofluorescence-FISH, the detection of FCCs was higher than that by light microscopy (24.0% vs. 2.0%). Samples were categorized into positive and negative groups, based on the expressions of CEP8, CEP17, and EpCAM. Statistically significant relationships were demonstrated between age (P = 0.029), sex (P = 0.002), lymphatic invasion (P = 0.001), pTNM stage (P = 0.001), and positivity for FCCs. After adjusting for covariates, patients with positive FCCs had lower progression-free survival than patients with negative FCCs.
UNASSIGNED: The ISET platform highly enriched nucleated cells from peritoneal lavage fluid, and indicators comprising EpCAM, CEP8, and CEP17 confirmed the diagnosis of FCCs. As a potential detection method, it offers an opportunity for early intervention of OPM and an extension of patient survival.
■通过上皮肿瘤细胞大小分离(ISET)方法,从50例GC患者中获得并处理了腹膜灌洗液。免疫荧光和荧光原位杂交(FISH)用于鉴定表达8号染色体(CEP8)的FCCs,染色体17(CEP17),上皮细胞粘附分子(EpCAM)。
■使用ISET平台和免疫荧光-FISH的组合,FCCs的检测高于光学显微镜(24.0%vs.2.0%)。样本分为阳性和阴性组,基于CEP8、CEP17和EpCAM的表达式。年龄之间具有统计学上的显着关系(P=0.029),性别(P=0.002),淋巴浸润(P=0.001),pTNM分期(P=0.001),和FCC的积极性。在调整协变量后,FCC阳性患者的无进展生存期低于FCC阴性患者.
■ISET平台从腹腔灌洗液中高度富集有核细胞,和包括EpCAM的指标,CEP8和CEP17证实了FCC的诊断。作为一种潜在的检测方法,它为OPM的早期干预和延长患者生存期提供了机会.