PDF(Pubmed)
Abstract：
UNASSIGNED: To investigate the relationship between the DNA methylation state of NRG1 promoter and its expression changes, and to analyze the clinical significance of its regulatory mechanism of DNA methylation in cervical carcinoma.
UNASSIGNED: This was a retrospective study. One-hundred and twenty patients from the Department of Gynecology of Cangzhou People\'s Hospital from September 2017 to September 2019 were selected, including 40 cases of cervical SCC, 40 cases of high grade squamous intraepithelial lesions(HSIL) and 40 cases of control cervical tissues. RT-qPCR, immunohistochemistry and DNA methylation-specific PCR(MSP) were used to detect the mRNA and protein expression of NRG1 and DNA methylation status in different tissue types.
UNASSIGNED: Immunohistochemical results showed that the positive protein expression rate of NRG1 gene in the SCC group was lower than that in both HSIL and Control groups. RT-qPCR results showed that the mRNA gene of NRG1 gradually decreased in expression with the increase of cervical tissue lesions, with a statistically significant difference. Similarly, it also found that the mRNA expression level of NRG1 in the SCC group was independent of patients\' age (p>0.05), but significantly correlated with tumor pathological staging, surgical pathology staging and lymphatic metastasis (p<0.05). Furthermore, methylation-specific PCR results revealed a significantly higher DNA methylation rate of NRG1 gene in the SCC group than in both HSIL and Control groups, with a statistically significant difference. Moreover, the methylation degree of NRG1 gene in SCC tissues was negatively correlated with its mRNA expression (p<0.05).
UNASSIGNED: Abnormal DNA hypermethylation of NRG1 gene inhibits the expression of mRNA and protein in the progression of cervical tissue from normal to cancerous state, which is involved in the occurrence and development of cervical carcinoma.
UNASSIGNED: This was a retrospective study. One-hundred and twenty patients from the Department of Gynecology of Cangzhou People\'s Hospital from September 2017 to September 2019 were selected, including 40 cases of cervical SCC, 40 cases of high grade squamous intraepithelial lesions(HSIL) and 40 cases of control cervical tissues. RT-qPCR, immunohistochemistry and DNA methylation-specific PCR(MSP) were used to detect the mRNA and protein expression of NRG1 and DNA methylation status in different tissue types.
UNASSIGNED: Immunohistochemical results showed that the positive protein expression rate of NRG1 gene in the SCC group was lower than that in both HSIL and Control groups. RT-qPCR results showed that the mRNA gene of NRG1 gradually decreased in expression with the increase of cervical tissue lesions, with a statistically significant difference. Similarly, it also found that the mRNA expression level of NRG1 in the SCC group was independent of patients\' age (p>0.05), but significantly correlated with tumor pathological staging, surgical pathology staging and lymphatic metastasis (p<0.05). Furthermore, methylation-specific PCR results revealed a significantly higher DNA methylation rate of NRG1 gene in the SCC group than in both HSIL and Control groups, with a statistically significant difference. Moreover, the methylation degree of NRG1 gene in SCC tissues was negatively correlated with its mRNA expression (p<0.05).
UNASSIGNED: Abnormal DNA hypermethylation of NRG1 gene inhibits the expression of mRNA and protein in the progression of cervical tissue from normal to cancerous state, which is involved in the occurrence and development of cervical carcinoma.
摘要：
■探讨NRG1启动子DNA甲基化状态与其表达变化的关系,并分析其在宫颈癌中DNA甲基化调控机制的临床意义。
■这是一项回顾性研究。选取沧州市人民医院2017年9月至2019年9月妇科患者120例，包括40例宫颈鳞癌，高级别鳞状上皮内病变(HSIL)40例,对照宫颈组织40例。RT-qPCR,免疫组织化学和DNA甲基化特异性PCR(MSP)检测不同组织类型NRG1的mRNA和蛋白表达及DNA甲基化状态。
■免疫组化结果显示，SCC组NRG1基因的阳性蛋白表达率低于HSIL组和对照组。RT-qPCR结果显示,随着宫颈组织病变的增加,NRG1的mRNA表达逐渐降低,具有统计学上的显著差异。同样，还发现SCC组中NRG1的mRNA表达水平与患者年龄无关（p>0.05），但与肿瘤病理分期显著相关,手术病理分期和淋巴结转移(p<0.05)。此外，甲基化特异性PCR结果显示SCC组NRG1基因的DNA甲基化率显著高于HSIL组和对照组,具有统计学上的显著差异。此外，SCC组织中NRG1基因甲基化程度与其mRNA表达呈负相关(p<0.05)。
■NRG1基因DNA异常甲基化抑制宫颈组织从正常到癌变过程中mRNA和蛋白的表达，参与了宫颈癌的发生发展。
■这是一项回顾性研究。选取沧州市人民医院2017年9月至2019年9月妇科患者120例，包括40例宫颈鳞癌，高级别鳞状上皮内病变(HSIL)40例,对照宫颈组织40例。RT-qPCR,免疫组织化学和DNA甲基化特异性PCR(MSP)检测不同组织类型NRG1的mRNA和蛋白表达及DNA甲基化状态。
■免疫组化结果显示，SCC组NRG1基因的阳性蛋白表达率低于HSIL组和对照组。RT-qPCR结果显示,随着宫颈组织病变的增加,NRG1的mRNA表达逐渐降低,具有统计学上的显著差异。同样，还发现SCC组中NRG1的mRNA表达水平与患者年龄无关（p>0.05），但与肿瘤病理分期显著相关,手术病理分期和淋巴结转移(p<0.05)。此外，甲基化特异性PCR结果显示SCC组NRG1基因的DNA甲基化率显著高于HSIL组和对照组,具有统计学上的显著差异。此外，SCC组织中NRG1基因甲基化程度与其mRNA表达呈负相关(p<0.05)。
■NRG1基因DNA异常甲基化抑制宫颈组织从正常到癌变过程中mRNA和蛋白的表达，参与了宫颈癌的发生发展。
