PDF(Pubmed)
Abstract：
UNASSIGNED: This study investigated the in vivo embryotoxicity, teratogenic potential, and additional effects of orthodontic acrylic resin as well as its components, utilizing zebrafish as a model organism. The research focused on morphological, cardiac, behavioral, and cognitive evaluations that were performed on embryos and larval-stage animals subjected to chronic exposure.
UNASSIGNED: Embryo and larval-stage zebrafish were categorized into five experimental groups, which were further subdivided into five subgroups. These subgroups included three specific doses for each tested substance, a control with the vehicle (0.1 % dimethyl sulfoxide in water), and an absolute control (water). Assessments were performed on day 5 post-fertilization, which included morphological, cardiac, behavioral, and cognitive evaluations. All experiments had a sample size of ten animals and were performed in triplicate. Survival and hatching rates were analyzed using the Kaplan-Meier test, while other measurements were assessed using one-way analysis of variance (ANOVA), followed by the Tukey post hoc test.
UNASSIGNED: Statistically significant differences were observed between the control and treatment groups across all the tested substances for heart rate, cognitive responsiveness, and cellular apoptosis. However, survival, hatching rate, and other parameters exhibited no significant variation, except for the highest dose in the dibutyl phthalate group, which demonstrated a notable difference in survival.
UNASSIGNED: Chronic exposure to acrylic resin and its components may be associated with decreased cognitive ability and cardiac rhythm, as well as an increase in the level of cellular apoptosis in zebrafish.
UNASSIGNED: Embryo and larval-stage zebrafish were categorized into five experimental groups, which were further subdivided into five subgroups. These subgroups included three specific doses for each tested substance, a control with the vehicle (0.1 % dimethyl sulfoxide in water), and an absolute control (water). Assessments were performed on day 5 post-fertilization, which included morphological, cardiac, behavioral, and cognitive evaluations. All experiments had a sample size of ten animals and were performed in triplicate. Survival and hatching rates were analyzed using the Kaplan-Meier test, while other measurements were assessed using one-way analysis of variance (ANOVA), followed by the Tukey post hoc test.
UNASSIGNED: Statistically significant differences were observed between the control and treatment groups across all the tested substances for heart rate, cognitive responsiveness, and cellular apoptosis. However, survival, hatching rate, and other parameters exhibited no significant variation, except for the highest dose in the dibutyl phthalate group, which demonstrated a notable difference in survival.
UNASSIGNED: Chronic exposure to acrylic resin and its components may be associated with decreased cognitive ability and cardiac rhythm, as well as an increase in the level of cellular apoptosis in zebrafish.
摘要：
■这项研究调查了体内胚胎毒性，致畸潜力，以及正畸丙烯酸树脂及其成分的附加作用，利用斑马鱼作为模型生物。研究集中在形态学上，心脏,行为，以及对长期暴露的胚胎和幼虫期动物进行的认知评估。
■胚胎和幼体阶段斑马鱼分为五个实验组，进一步细分为五个子组。这些亚组包括每种测试物质的三个特定剂量，与车辆的对照（水中0.1%二甲基亚砜），和绝对控制（水）。在受精后第5天进行评估，其中包括形态学，心脏,行为，和认知评估。所有实验具有10只动物的样品大小,并且一式三份进行。使用Kaplan-Meier检验分析存活率和孵化率，而其他测量值使用单向方差分析(ANOVA)进行评估，其次是Tukey事后测试。
■在所有测试物质的心率方面，对照组和治疗组之间观察到统计学上的显着差异，认知反应，和细胞凋亡。然而,生存,孵化率,和其他参数没有表现出显著的变化，除了邻苯二甲酸二丁酯组的最高剂量，这证明了存活率的显著差异。
■长期接触丙烯酸树脂及其成分可能与认知能力和心律下降有关，以及斑马鱼细胞凋亡水平的增加。
■胚胎和幼体阶段斑马鱼分为五个实验组，进一步细分为五个子组。这些亚组包括每种测试物质的三个特定剂量，与车辆的对照（水中0.1%二甲基亚砜），和绝对控制（水）。在受精后第5天进行评估，其中包括形态学，心脏,行为，和认知评估。所有实验具有10只动物的样品大小,并且一式三份进行。使用Kaplan-Meier检验分析存活率和孵化率，而其他测量值使用单向方差分析(ANOVA)进行评估，其次是Tukey事后测试。
■在所有测试物质的心率方面，对照组和治疗组之间观察到统计学上的显着差异，认知反应，和细胞凋亡。然而,生存,孵化率,和其他参数没有表现出显著的变化，除了邻苯二甲酸二丁酯组的最高剂量，这证明了存活率的显著差异。
■长期接触丙烯酸树脂及其成分可能与认知能力和心律下降有关，以及斑马鱼细胞凋亡水平的增加。
