PDF(Pubmed)
Abstract：
BACKGROUND: Sarcocystis is a food-borne zoonotic protozoan whose final hosts are humans, dogs, cats, and other carnivores and intermediate hosts are birds and mammals, especially humans and herbivores. Humans become infected by eating raw and undercooked meat contaminated with bradyzoites or by consuming water or food contaminated with the sporocyst stage of the parasite.
OBJECTIVE: The aim of this study was to investigate the effects of gamma radiation and electron beam on the survival rate of Sarcocystis bradyzoites in infected beef and to determine the effective dose.
METHODS: Three replicates of 100 g of infected meat were treated with different doses (0.5, 1, 1.5 and 2 kGy). As a control, 20 g of contaminated meat was stored separately at 4°C. The viability of the bradyzoites after digestion in pepsin solution was assessed, stained (trypan blue) and unstained, under a stereomicroscope. To assess survival of the bradyzoites, the irradiated meat samples were fed to 30 dogs. After 10 days, faecal samples were examined for sporocysts.
RESULTS: The results showed that the highest and lowest mortality rate of Sarcocystis bradyzoites in infected organs using electron beam at a dose of 2 kGy were 92.5% and 100%, respectively, and the lowest mortality rate at a dose of 0.5 kGy were 2.5% and 7.89%, respectively.
CONCLUSIONS: The results of statistical analysis showed that the mortality rate of Sarcocystis bradyzoites was significant between different doses of gamma ray and electron beam, so that gamma rays were better compared to electron beam in destroying Sarcocystis bradyzoites.
OBJECTIVE: The aim of this study was to investigate the effects of gamma radiation and electron beam on the survival rate of Sarcocystis bradyzoites in infected beef and to determine the effective dose.
METHODS: Three replicates of 100 g of infected meat were treated with different doses (0.5, 1, 1.5 and 2 kGy). As a control, 20 g of contaminated meat was stored separately at 4°C. The viability of the bradyzoites after digestion in pepsin solution was assessed, stained (trypan blue) and unstained, under a stereomicroscope. To assess survival of the bradyzoites, the irradiated meat samples were fed to 30 dogs. After 10 days, faecal samples were examined for sporocysts.
RESULTS: The results showed that the highest and lowest mortality rate of Sarcocystis bradyzoites in infected organs using electron beam at a dose of 2 kGy were 92.5% and 100%, respectively, and the lowest mortality rate at a dose of 0.5 kGy were 2.5% and 7.89%, respectively.
CONCLUSIONS: The results of statistical analysis showed that the mortality rate of Sarcocystis bradyzoites was significant between different doses of gamma ray and electron beam, so that gamma rays were better compared to electron beam in destroying Sarcocystis bradyzoites.
摘要：
背景：肉囊虫是一种食源性的人畜共患原生动物，其最终宿主是人类，狗,猫,其他食肉动物和中间宿主是鸟类和哺乳动物，尤其是人类和食草动物.人类通过食用被缓子污染的生肉和未煮熟的肉或通过食用被寄生虫孢子囊期污染的水或食物而感染。
目的：本研究的目的是研究γ射线和电子束对感染牛肉中节育孢子虫成活率的影响，并确定有效剂量。
方法：用不同剂量（0.5、1、1.5和2kGy）处理100g感染肉的三个重复。作为一种控制，将20g受污染的肉在4°C下单独储存。在胃蛋白酶溶液中消化后，评估了缓生子的活力，染色（锥虫蓝）和未染色，在立体显微镜下.为了评估缓生子的生存，经辐照的肉样被饲喂30只狗。10天后,检查粪便样品中的孢子囊。
结果：结果表明，以2kGy的剂量使用电子束在感染器官中的节食孢子虫的最高和最低死亡率分别为92.5％和100％，分别,0.5kGy剂量的最低死亡率分别为2.5%和7.89%,分别。
结论：统计分析结果表明，不同剂量的伽马射线和电子束下，节肢动物死亡率差异显著，因此，与电子束相比，伽马射线更好地破坏了肉孢子虫。
目的：本研究的目的是研究γ射线和电子束对感染牛肉中节育孢子虫成活率的影响，并确定有效剂量。
方法：用不同剂量（0.5、1、1.5和2kGy）处理100g感染肉的三个重复。作为一种控制，将20g受污染的肉在4°C下单独储存。在胃蛋白酶溶液中消化后，评估了缓生子的活力，染色（锥虫蓝）和未染色，在立体显微镜下.为了评估缓生子的生存，经辐照的肉样被饲喂30只狗。10天后,检查粪便样品中的孢子囊。
结果：结果表明，以2kGy的剂量使用电子束在感染器官中的节食孢子虫的最高和最低死亡率分别为92.5％和100％，分别,0.5kGy剂量的最低死亡率分别为2.5%和7.89%,分别。
结论：统计分析结果表明，不同剂量的伽马射线和电子束下，节肢动物死亡率差异显著，因此，与电子束相比，伽马射线更好地破坏了肉孢子虫。
