PDF(Pubmed)
Abstract：
OBJECTIVE: Livestock droppings cause some environmental problems, but they have the potential to be used as effective biomass resources. The black soldier fly (BSF), Hermetia illucens (Diptera: Stratiomyidae), is suitable for efficiently processing such resources. By using BSF larvae for the disposal of livestock droppings, we can obtain two valuable products: protein resources and organic fertilizer. However, there is insufficient research on the digestive enzymes suitable for processing this waste. Here, we aimed to construct an efficient BSF processing system using livestock droppings, and we explored the digestive enzymes involved in this process.
RESULTS: First, we investigated the characteristics of transcripts expressed in the midgut of BSF larvae and found that immune response-related genes were expressed in the midgut. Then, we investigated digestive enzymes and identified a novel serine protease, HiBrachyurin, whose mRNA was highly expressed in the posterior midgut when BSF larvae fed on horse droppings. Despite the low protein content of horse droppings, larvae that fed on horse droppings accumulated more protein than those in the other groups. Therefore, HiBrachyurin may contribute to digestibility in the early stage of protein degradation in BSF larvae fed on horse droppings.
RESULTS: First, we investigated the characteristics of transcripts expressed in the midgut of BSF larvae and found that immune response-related genes were expressed in the midgut. Then, we investigated digestive enzymes and identified a novel serine protease, HiBrachyurin, whose mRNA was highly expressed in the posterior midgut when BSF larvae fed on horse droppings. Despite the low protein content of horse droppings, larvae that fed on horse droppings accumulated more protein than those in the other groups. Therefore, HiBrachyurin may contribute to digestibility in the early stage of protein degradation in BSF larvae fed on horse droppings.
摘要：
目的：家畜粪便会引起一些环境问题，但它们有潜力被用作有效的生物质资源。黑人士兵飞（BSF）隐虫（双翅目：层状科），适用于有效处理此类资源。通过使用BSF幼虫处理牲畜粪便，我们可以获得两种有价值的产品:蛋白质资源和有机肥料。然而,对适合处理这种废物的消化酶研究不足。这里,我们的目标是利用牲畜粪便构建一个高效的BSF处理系统，我们探索了这个过程中涉及的消化酶。
结果：首先，我们研究了BSF幼虫中肠表达的转录本的特征，发现免疫反应相关基因在中肠表达。然后，我们研究了消化酶并鉴定了一种新的丝氨酸蛋白酶，HiBrachyurin,当BSF幼虫以马粪为食时，其mRNA在后中肠中高表达。尽管马粪的蛋白质含量很低，以马粪为食的幼虫比其他组的幼虫积累了更多的蛋白质。因此,HiBrachyurin可能有助于以马粪便为食的BSF幼虫在蛋白质降解的早期阶段的消化率。
结果：首先，我们研究了BSF幼虫中肠表达的转录本的特征，发现免疫反应相关基因在中肠表达。然后，我们研究了消化酶并鉴定了一种新的丝氨酸蛋白酶，HiBrachyurin,当BSF幼虫以马粪为食时，其mRNA在后中肠中高表达。尽管马粪的蛋白质含量很低，以马粪为食的幼虫比其他组的幼虫积累了更多的蛋白质。因此,HiBrachyurin可能有助于以马粪便为食的BSF幼虫在蛋白质降解的早期阶段的消化率。
