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Abstract：
The cotton whitefly, Bemisia tabaci, is considered as a species complex with 46 cryptic species, with Asia II-1 being predominant in Asia. This study addresses a significant knowledge gap in the characterization of odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) in Asia II-1. We explored the expression patterns of OBPs and CSPs throughout their developmental stages and compared the motif patterns of these proteins. Significant differences in expression patterns were observed for the 14 OBPs and 14 CSPs of B. tabaci Asia II-1, with OBP8 and CSP4 showing higher expression across the developmental stages. Phylogenetic analysis reveals that OBP8 and CSP4 form distinct clades, with OBP8 appearing to be an ancestral gene, giving rise to the evolution of other odorant-binding proteins in B. tabaci. The genomic distribution of OBPs and CSPs highlights gene clustering on the chromosomes, suggesting functional conservation and evolutionary events following the birth-and-death model. Molecular docking studies indicate strong binding affinities of OBP8 and CSP4 with various odour compounds like β-caryophyllene, α-pinene, β-pinene and limonene, reinforcing their roles in host recognition and reproductive functions. This study elaborates on our understanding of the putative roles of different OBPs and CSPs in B. tabaci Asia II-1, hitherto unexplored. The dynamics of the expression of OBPs and CSPs and their interactions with odour compounds offer scope for developing innovative methods for controlling this global invasive pest.
摘要：
棉粉虱，烟粉虱,被认为是一个有46个神秘物种的物种复合体，亚洲II-1在亚洲占主导地位。这项研究解决了亚洲II-1中气味结合蛋白（OBP）和化学感应蛋白（CSP）表征方面的重大知识空白。我们探索了OBP和CSP在整个发育阶段的表达模式，并比较了这些蛋白质的基序模式。对于烟粉虱亚洲II-1的14个OBP和14个CSP，观察到表达模式的显着差异，OBP8和CSP4在整个发育阶段显示出更高的表达。系统发育分析表明OBP8和CSP4形成不同的进化枝，OBP8似乎是祖先基因，引起烟粉虱中其他气味结合蛋白的进化。OBP和CSP的基因组分布突出了染色体上的基因聚类，提示出生和死亡模型后的功能保守和进化事件。分子对接研究表明OBP8和CSP4与各种气味化合物如β-石竹烯的强结合亲和力，α-pine烯,β-pine烯和柠檬烯，加强它们在宿主识别和生殖功能中的作用。这项研究详细阐述了我们对迄今为止尚未探索的不同OBP和CSP在B.tabaciAsiaII-1中的假定作用的理解。OBP和CSP的表达动态及其与气味化合物的相互作用为开发控制这种全球入侵害虫的创新方法提供了空间。
