关键词: Baculovirus expression vector system (BEVS) High titer stock (HTS) Hours post infection (hpi) Initial virus seed stock (P0) Insect cell culture Optimization Protein expression Scale-up Stirred tank reactor (STR)

Mesh : Baculoviridae / genetics Recombinant Proteins / genetics biosynthesis Genetic Vectors / genetics Animals Humans Sf9 Cells

来  源:   DOI:10.1007/978-1-0716-3961-0_13

Abstract:
The Baculovirus Expression Vector System (BEVS) has revolutionized the field of recombinant protein expression by enabling efficient and high yield production. The platform offers many advantages including manufacturing speed, flexible design, and scalability. In this chapter, we describe the methods including strategies and considerations to successfully optimize and scale-up using BEVS as a tool for production (Fig. 1). As an illustrative case study, we present an example focused on the production of a viral glycoprotein.
摘要:
杆状病毒表达载体系统(BEVS)通过实现高效和高产量的生产,彻底改变了重组蛋白表达领域。该平台提供了许多优势,包括制造速度,灵活的设计,和可扩展性。在这一章中,我们描述了使用BEVS作为生产工具成功优化和扩大规模的方法,包括策略和注意事项(图。1).作为一个说明性的案例研究,我们提供了一个例子,重点是病毒糖蛋白的生产。
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