Abstract：
The aim of this study was to evaluate the influence of liver fibrosis (LF) on the expression of Toll-like receptors (TLR) 2 and 4 in apical periodontitis (AP) in Wistar rats. Forty Wistar rats were allocated in the following groups (n = 10): C-control; AP-apical periodontitis; LF-liver fibrosis; AP + LF-rats with AP and LF. LF and AP were induced by established methodologies. Histological, bacteriological, and immunohistochemical analyses were performed according to pre-established scores. For comparisons between AP and AP + LF groups, the Mann-Whitney test was used (P < .05). The livers of the LF and AP + LF groups showed generalized portal inflammatory infiltrate and collagen fibers confirming the presence of LF. Histopathological analysis in the maxilla of the AP + LF group showed areas of necrosis comprising the entire dental pulp and periapical tissue surrounded by a more intense inflammatory infiltrate than observed in the AP group (P = 0.032). A significant number of specimens in the AP + LF group showed microorganisms beyond the apical foramen adhered to the extraradicular biofilm, demonstrating greater invasion compared to the AP group (P = .008). Immunohistochemical analysis showed a large number of cells immunoreactive for TLR2 and TLR4 in the AP + LF group, compared to the AP group (P < 0.05). Liver fibrosis favors the inflammation and contamination of microorganisms in apical periodontitis and triggers the expression of TLR2 and TLR4, modulating innate immunity response in periapical lesions.
摘要：
本研究的目的是评估肝纤维化（LF）对Wistar大鼠根尖周炎（AP）中Toll样受体（TLR）2和4表达的影响。将40只Wistar大鼠分为以下组（n=10）：C对照；AP根尖周炎；LF肝纤维化；AP和LF的APLF大鼠。通过已建立的方法诱导LF和AP。组织学,细菌学，根据预先建立的评分进行免疫组织化学分析.对于AP和AP+LF组之间的比较，采用Mann-Whitney检验(P<0.05)。LF和AP+LF组的肝脏显示出广泛的门静脉炎性浸润和胶原纤维,证实存在LF。APLF组上颌骨的组织病理学分析显示，与AP组相比，坏死区域包括整个牙髓和根尖周组织，周围有更强烈的炎症浸润（P=0.032）。AP+LF组的大量标本显示根尖孔以外的微生物粘附在针状外生物膜上，与AP组相比，显示出更大的侵袭(P=.008)。免疫组织化学分析显示,AP+LF组有大量TLR2和TLR4免疫反应的细胞,与AP组比较(P<0.05)。肝纤维化有利于根尖周炎中微生物的炎症和污染，并触发TLR2和TLR4的表达，从而调节根尖周炎中的固有免疫反应。
