Abstract：
A Gram-stain-negative, aerobic, rod-shaped and motile strain HL-JVS1T, was isolated from the gastric tract of a juvenile Pacific white shrimp. Molecular phylogenetic analysis based on 16S rRNA gene sequences of strain HL-JVS1T revealed its affiliation with the genus Pleionea, with close relatives including Pleionea mediterranea MOLA115T (97.5%) and Pleionea sediminis S1-5-21T (96.2%). The complete genome of strain HL-JVS1T consisted of a circular 4.4 Mb chromosome and two circular plasmids (6.6 and 35.0 kb) with a G + C content of 43.1%. The average nucleotide identity and digital DNA-DNA hybridization values between strain HL-JVS1T and the type strains of described Pleionea species were 69.7-70.4% and 18.3-18.6%, respectively. Strain HL-JVS1T grew at 10-40 °C (optimum, 30 °C) in the presence of 0.5 - 9.0% (w/v) sea salts (optimum, 2.0 - 2.5%), and at pH range of 5.5 - 10.0 (optimum, pH 6.5). The major fatty acids (> 10%) were summed feature 9 (iso-C17:1 ω9c and/or C16:0 10-methyl) (23.3%), iso-C16:0 (14.5%), iso-C11:0 3-OH (13.8%) and iso-C15:0 (11.0%). The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an unidentified aminophospholipid, two unidentified aminolipids, and two unidentified lipids. The respiratory quinone was ubiquinone-8. The comprehensive phylogenetic, phylogenomic, phenotypic and chemotaxonomic results showed that strain HL-JVS1T is distinct from other Pleionea species. Hence, we propose strain HL-JVS1T as a novel species belonging to the genus Pleionea, for which the name Pleionea litopenaei sp. nov. is proposed with HL-JVS1T (= KCCM 90514T = JCM 36490T) as the type strain.
摘要：
革兰氏染色阴性，有氧,杆状和活动应变HL-JVS1T，是从幼年太平洋白虾的胃道中分离出来的。基于HL-JVS1T菌株16SrRNA基因序列的分子系统发育分析揭示了其与Pleionea属的隶属关系，近亲包括地中海PleioneaMOLA115T（97.5％）和地中海PleioneaS1-5-21T（96.2％）。菌株HL-JVS1T的完整基因组由环状4.4Mb染色体和两个环状质粒（6.6和35.0kb）组成，GC含量为43.1％。HL-JVS1T菌株与所述Pleionea物种的类型菌株之间的平均核苷酸同一性和数字DNA-DNA杂交值分别为69.7-70.4％和18.3-18.6％，分别。菌株HL-JVS1T在10-40°C生长(最佳，30°C）在0.5-9.0％（w/v）海盐存在下（最佳，2.0-2.5%），在5.5-10.0的pH范围内(最佳，pH6.5)。主要脂肪酸（>10％）的总和特征为9（异C17：1ω9c和/或C16：010-甲基）（23.3％），iso-C16:0（14.5%），iso-C11:03-OH(13.8%)和iso-C15:0(11.0%)。极性脂质是磷脂酰乙醇胺，磷脂酰甘油，二磷脂酰甘油，一种身份不明的氨基磷脂,两种身份不明的氨基脂类,和两种身份不明的脂质.呼吸醌是泛醌-8。全面的系统发育，系统发育，表型和化学分类学结果表明，菌株HL-JVS1T与其他Pleionea物种不同。因此，我们提出菌株HL-JVS1T是属于Pleionea属的新物种，其名称为Pleionealitopenaeisp.11月。以HL-JVS1T（=KCCM90514T=JCM36490T）为类型应变。
