Abstract：
A Gram-negative, aerobic, rod-shaped, non-motile bacterium, designated as FTW29T, was isolated from surface seawater sampled in Futian district, Shenzhen, China. Growth of strain FTW29T was observed at 15-42 ℃ (optimum, 28-30 ℃), pH 4.0-9.0 (optimum, pH 5.5-7.5) and in the presence of 0.5-10% NaCl (optimum, 3.0% NaCl). Strain FTW29T showed 95.0-96.8% 16 S rRNA gene sequence similarity to various type strains of the genera Thioclava, Sinirhodobacter, Rhodobacter, Haematobacter and Frigidibacter of the family Paracoccaceae, and its most closely related strains were Thioclava pacifica DSM 10,166T (96.8%) and Thioclava marina 11.10-0-13T (96.7%). The phylogenomic tree constructed on the bac120 gene set showed that strain FTW29T formed a clade with the genus Thioclava, with a bootstrap value of 100%. The evolutionary distance values between FTW29T and type strains of the genus Thioclava were 0.17-0.19, which are below the recommended standard (0.21-0.23) for defining a novel genus in the family Paracoccaceae. In strain FTW29T, the major fatty acids identified were summed feature 8 (C18:1ω7c) and C16:0, and the predominant respiratory quinones were ubiquinone-10 and ubiquinone-9. The composition of polar lipids in strain FTW29T included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid, an unidentified aminolipid, two unidentified glycolipids and an unidentified lipid. The genome of strain FTW29T comprised one circle chromosome and six plasmids, with a G + C content of 61.4%. The average nucleotide identity, average amino acid identity, and digital DNA-DNA hybridization values between strain FTW29T and seven type strains of the genus Thioclava were 76.6-78.4%, 53.2-56.4% and 19.3-20.4%, respectively. Altogether, the phenotypic, phylogenetic and chemotaxonomic evidence illustrated in this study suggested that strain FTW29T represents a novel species of the genus Thioclava, with the proposed name Thioclava litoralis sp. nov. The type strain is FTW29T (= KCTC 82,841T = MCCC 1K08523T).
摘要：
革兰氏阴性，有氧,杆状,不运动的细菌，指定为FTW29T，从福田区采样的地表海水中分离出来，深圳,中国。菌株FTW29T的生长在15-42℃(最佳，28-30℃),pH4.0-9.0（最佳，pH5.5-7.5），并且在0.5-10％NaCl存在下（最佳，3.0%NaCl)。菌株FTW29T与硫克拉瓦属的各种类型菌株显示出95.0-96.8％的16SrRNA基因序列相似性，西尼氏细菌,红杆菌属,副杆菌属的不动杆菌和寒杆菌，与其最密切相关的菌株是太平洋硫脲DSM10,166T（96.8％）和太平洋硫脲码头11.10-0-13T（96.7％）。在bac120基因集上构建的系统发育树表明，菌株FTW29T与硫克拉瓦属形成了进化枝，引导值为100%。FTW29T与Thioclava属的类型菌株之间的进化距离值为0.17-0.19，低于定义Paracoccaceae科新属的推荐标准（0.21-0.23）。在菌株FTW29T中，确定的主要脂肪酸为特征8（C18：1ω7c）和C16：0，主要的呼吸醌是泛醌10和泛醌9。菌株FTW29T中极性脂质的组成包括二磷脂酰甘油，磷脂酰甘油，磷脂酰乙醇胺，一种身份不明的磷脂,一种身份不明的氨基脂质,两种身份不明的糖脂和一种身份不明的脂质。菌株FTW29T的基因组包含一个环状染色体和六个质粒，G+C含量为61.4%。平均核苷酸同一性，平均氨基酸同一性，菌株FTW29T与7种硫属菌株之间的数字DNA-DNA杂交值为76.6-78.4％，53.2-56.4%和19.3-20.4%，分别。总之,表型，本研究中说明的系统发育和化学分类学证据表明，菌株FTW29T代表了硫克拉瓦属的一种新物种，拟议名称为Thioclavalitoralissp.11月。菌株类型为FTW29T(=KCTC82,841T=MCCC1K08523T)。
