Abstract：
BACKGROUND: Shegan-Mahuang Decoction (SMD) is a classical formula that has been used to effectively treat cold-induced asthma (CA) for 1800 years. Airway smooth muscle cells (ASMCs) play a crucial role in airway remodeling of CA and can be modulated through bitter taste-sensing type 2 receptors (TAS2Rs). Given that SMD contains numerous bitter herbs and TAS2R10 expression in ASMCs remains consistently high, it is pertinent to explore whether SMD regulates ASMCs via TAS2R10 to exert its CA mechanism.
OBJECTIVE: This study investigated the efficacy as well as the potential mechanism of SMD in CA.
METHODS: In this study, experiments in vivo were conducted using the CA rat model induced by ovalbumin (OVA) along with cold stimulation. The effects of SMD and TAS2R10 expression in CA rats were evaluated using the following methods: clinical symptoms, weights, pathological staining, immunofluorescence staining (IF), enzyme-linked immunosorbent assay (ELISA), real-time quantitative polymerase chain reaction (RT-qPCR) and Western blot (WB). Assays in vitro including cell counting Kit-8 (CCK-8), ELISA, flow cytometry, TUNEL staining, RT-qPCR and WB were performed to investigate potential mechanism of SMD on the proliferation and apoptosis of ASMCs through upregulation of TAS2R10.
RESULTS: The administration of SMD resulted in a notable improvement in the symptoms, trends in weight, airway inflammation and airway remodeling observed in CA rats with upregulated TAS2R10. Mechanistically, we furtherly confirmed that SMD inhibits p70S6K/CyclinD1 pathway by upregulating TAS2R10. SMD furthermore blocked the G0/G1 phase, suppressed the proliferation and inducted apoptosis in ASMCs induced by platelet-derived growth factor-BB (PDGF-BB). Erythromycin (EM), a TAS2R10 agonist, can intensify these effects.
CONCLUSIONS: SMD significantly ameliorates CA by upregulating TAS2R10 and inhibiting the p70S6K/CyclinD1 pathway, thereby modulating ASMCs\' proliferation and apoptosis. Inspired by the Five Flavors Theory of Traditional Chinese Medicine, this study provides an updated treatment perspective for treating CA.
OBJECTIVE: This study investigated the efficacy as well as the potential mechanism of SMD in CA.
METHODS: In this study, experiments in vivo were conducted using the CA rat model induced by ovalbumin (OVA) along with cold stimulation. The effects of SMD and TAS2R10 expression in CA rats were evaluated using the following methods: clinical symptoms, weights, pathological staining, immunofluorescence staining (IF), enzyme-linked immunosorbent assay (ELISA), real-time quantitative polymerase chain reaction (RT-qPCR) and Western blot (WB). Assays in vitro including cell counting Kit-8 (CCK-8), ELISA, flow cytometry, TUNEL staining, RT-qPCR and WB were performed to investigate potential mechanism of SMD on the proliferation and apoptosis of ASMCs through upregulation of TAS2R10.
RESULTS: The administration of SMD resulted in a notable improvement in the symptoms, trends in weight, airway inflammation and airway remodeling observed in CA rats with upregulated TAS2R10. Mechanistically, we furtherly confirmed that SMD inhibits p70S6K/CyclinD1 pathway by upregulating TAS2R10. SMD furthermore blocked the G0/G1 phase, suppressed the proliferation and inducted apoptosis in ASMCs induced by platelet-derived growth factor-BB (PDGF-BB). Erythromycin (EM), a TAS2R10 agonist, can intensify these effects.
CONCLUSIONS: SMD significantly ameliorates CA by upregulating TAS2R10 and inhibiting the p70S6K/CyclinD1 pathway, thereby modulating ASMCs\' proliferation and apoptosis. Inspired by the Five Flavors Theory of Traditional Chinese Medicine, this study provides an updated treatment perspective for treating CA.
摘要：
背景：射干麻黄汤（SMD）是一种经典的配方，已用于有效治疗寒冷诱发的哮喘（CA）1800年。气道平滑肌细胞（ASMC）在CA的气道重塑中起着至关重要的作用，并且可以通过苦味感应2型受体（TAS2R）进行调节。鉴于SMD含有许多苦味草药，ASMC中的TAS2R10表达始终很高，探讨SMD是否通过TAS2R10调节ASMC以发挥其CA机制。
目的：本研究探讨了SMD在CA中的疗效和潜在机制。
方法：在本研究中，使用卵清蛋白（OVA）诱导的CA大鼠模型进行体内实验。使用以下方法评估CA大鼠SMD和TAS2R10表达的影响：临床症状，重量,病理染色，免疫荧光染色（IF），酶联免疫吸附测定(ELISA),实时定量聚合酶链反应（RT-qPCR）和蛋白质印迹（WB）。体外检测包括细胞计数试剂盒-8(CCK-8)，ELISA,流式细胞术,TUNEL染色,通过RT-qPCR和WB研究SMD通过上调TAS2R10对ASMC增殖和凋亡的潜在机制。
结果：使用SMD可显著改善症状，体重趋势，在TAS2R10上调的CA大鼠中观察到的气道炎症和气道重塑。机械上,我们进一步证实SMD通过上调TAS2R10抑制p70S6K/CyclinD1通路。SMD还阻断了G0/G1相,抑制血小板源性生长因子-BB(PDGF-BB)诱导的ASMCs增殖和诱导凋亡。红霉素（EM），一种TAS2R10激动剂，可以加强这些影响。
结论：SMD通过上调TAS2R10和抑制p70S6K/CyclinD1通路显著改善CA，从而调节ASMC的增殖和凋亡。受中医五味理论的启发，本研究为CA的治疗提供了一个更新的治疗视角.
目的：本研究探讨了SMD在CA中的疗效和潜在机制。
方法：在本研究中，使用卵清蛋白（OVA）诱导的CA大鼠模型进行体内实验。使用以下方法评估CA大鼠SMD和TAS2R10表达的影响：临床症状，重量,病理染色，免疫荧光染色（IF），酶联免疫吸附测定(ELISA),实时定量聚合酶链反应（RT-qPCR）和蛋白质印迹（WB）。体外检测包括细胞计数试剂盒-8(CCK-8)，ELISA,流式细胞术,TUNEL染色,通过RT-qPCR和WB研究SMD通过上调TAS2R10对ASMC增殖和凋亡的潜在机制。
结果：使用SMD可显著改善症状，体重趋势，在TAS2R10上调的CA大鼠中观察到的气道炎症和气道重塑。机械上,我们进一步证实SMD通过上调TAS2R10抑制p70S6K/CyclinD1通路。SMD还阻断了G0/G1相,抑制血小板源性生长因子-BB(PDGF-BB)诱导的ASMCs增殖和诱导凋亡。红霉素（EM），一种TAS2R10激动剂，可以加强这些影响。
结论：SMD通过上调TAS2R10和抑制p70S6K/CyclinD1通路显著改善CA，从而调节ASMC的增殖和凋亡。受中医五味理论的启发，本研究为CA的治疗提供了一个更新的治疗视角.
