Abstract：
A field population of Chrysoperla carnea was exposed for 17 generations to chlorfenapyr insecticide that resulted in 217-fold resistance compared to a susceptible strain. The overlapping of LC50 values in reciprocal crosses and their dominance values indicated that chlorfenapyr resistance was autosomal and incompletely dominant. The chi-square analysis of back-cross mortality confirmed the polygenic nature of chlorfenapyr resistance. The results of effective dominance of chlorfenapyr resistance indicated that resistance at the highest concentration was completely recessive. The realized heritability of chlorfenapyr resistance in the first 9, last 9, and a total of 18 generations was 0.28, 0.42, and 0.31, respectively. Furthermore, synergism results showed that both experimental synergists, PBO and DEF, did not synergize the toxicity of chlorfenapyr. In conclusion, C. carnea had been found to have autosomal, partially dominant, and polygenic chlorfenapyr resistance. Meaning that thereby resistance is inherited through multiple genes and is not limited to a single gene or sex-linked trait. These findings will help to develop an effective IPM model focusing on the simultaneous use of selective insecticides and resistant biocontrol agents to reduce the problem of resistance development in pest populations.
摘要：
将Chrysoperlacarnea的田间种群暴露于氯杀昆虫剂17代，与易感菌株相比，产生了217倍的抗性。倒数杂交中LC50值的重叠及其优势值表明，氯非那霉素抗性是常染色体和不完全显性的。回交死亡率的卡方分析证实了氯非那霉素抗性的多基因性质。氯非那吡菌属抗性有效优势的结果表明，在最高浓度下的抗性是完全隐性的。在前9代，后9代和总共18代的氯fenfenapyr抗性的实现遗传力分别为0.28、0.42和0.31。此外，协同作用结果表明，两种实验增效剂，PBO和DEF,没有协同毒害的毒性。总之,C.carnea被发现有常染色体，部分占优势,和多基因抗氯虫腈。这意味着抗性是通过多个基因遗传的,并且不限于单个基因或性连锁性状。这些发现将有助于开发一种有效的IPM模型，该模型着重于同时使用选择性杀虫剂和抗性生物防治剂，以减少害虫种群中抗性发展的问题。
