Abstract：
N6-methyladenosine (m6A) is a fundamentally important RNA modification for gene regulation, whose function is achieved through m6A readers. However, whether and how m6A readers play regulatory roles during fruit ripening and quality formation remains unclear. Here, we characterized SlYTH2 as a tomato m6A reader protein and profiled the binding sites of SlYTH2 at the transcriptome-wide level. SlYTH2 undergoes liquid-liquid phase separation and promotes RNA-protein condensate formation. The target mRNAs of SlYTH2, namely m6A-modified SlHPL and SlCCD1B associated with volatile synthesis, are enriched in SlYTH2-induced condensates. Through polysome profiling assays and proteomic analysis, we demonstrate that knockout of SlYTH2 expedites the translation process of SlHPL and SlCCD1B, resulting in augmented production of aroma-associated volatiles. This aroma enrichment significantly increased consumer preferences for CRISPR-edited fruit over wild type. These findings shed light on the underlying mechanisms of m6A in plant RNA metabolism and provided a promising strategy to generate fruits that are more attractive to consumers.
摘要：
N6-甲基腺苷（m6A）是基因调控的重要RNA修饰，其功能是通过m6A阅读器实现的。然而,m6A阅读器在果实成熟和品质形成过程中是否以及如何发挥调节作用尚不清楚。这里,我们将SlYTH2定性为番茄m6A阅读蛋白,并在全转录组水平对SlYTH2的结合位点进行了分析.SlYTH2经历液-液相分离并促进RNA-蛋白质缩合物的形成。SlYTH2的靶mRNA，即与挥发性合成相关的m6A修饰的SlHPL和SlCCD1B，富含SlYTH2诱导的冷凝物。通过多聚体谱分析和蛋白质组学分析，我们证明了SlYTH2的敲除加速了SlHPL和SlCCD1B的翻译过程，导致香气相关挥发物的产量增加。与野生型相比，这种香气富集显着增加了消费者对CRISPR编辑水果的偏好。这些发现揭示了m6A在植物RNA代谢中的潜在机制，并提供了一种有希望的策略来产生对消费者更具吸引力的水果。
