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Abstract：
Laryngeal squamous cell carcinoma (LSCC) is one of the most aggressive cancers that affect the head and neck region. Recent researches have confirmed that long non-coding RNAs (lncRNAs) present an emerging role in diversiform diseases including cancers. Prostate cancer-associated ncRNA transcript 6 (PCAT6) is an oncogene in lung cancer, cervical cancer, colon cancer and gastric cancer, but its role in LSCC is still unknown. In the current study, we attempted to figure out the role of PCAT6 in LSCC. RT-qPCR was to analyze PCAT6 expression in LSCC cells. Functional assays were to uncover the role of PCAT6 in LSCC. Mechanism assays were to explore the regulatory mechanism behind PCAT6 in LSCC. PCAT6 exhibited higher expression in LSCC cells and PCAT6 strengthened cell proliferation and inhibited cell apoptosis. Furthermore, lncRNA PCAT6 modulated notch receptor 3 expression and activated NOTCH signaling pathway via serving as a sponge for miR-4731-5p. Taken together, lncRNA PCAT6 was identified as an oncogene in LSCC, which revealed that PCAT6 might be used as potential therapeutic target for LSCC.
摘要：
喉鳞状细胞癌（LSCC）是影响头颈部区域的最具侵袭性的癌症之一。最近的研究已经证实，长链非编码RNA（lncRNAs）在包括癌症在内的多种疾病中发挥了新的作用。前列腺癌相关ncRNA转录本6（PCAT6）是肺癌的癌基因，宫颈癌,结肠癌和胃癌，但其在LSCC中的作用尚不清楚。在目前的研究中,我们试图弄清楚PCAT6在LSCC中的作用。RT-qPCR分析PCAT6在LSCC细胞中的表达。功能测定旨在揭示PCAT6在LSCC中的作用。机制实验旨在探索PCAT6在LSCC中的调控机制。PCAT6在LSCC细胞中表达较高，PCAT6增强细胞增殖，抑制细胞凋亡。此外，lncRNAPCAT6通过充当miR-4731-5p的海绵来调节notch受体3的表达并激活NOTCH信号通路。一起来看,lncRNAPCAT6在LSCC中被鉴定为癌基因，这表明PCAT6可能作为LSCC的潜在治疗靶点。
