Abstract：
Fungi occupy important environmental, cultural, and socioeconomic roles. However, biological research of this diverse kingdom has lagged behind that of other phylogenetic groups. This is partially the result of the notorious difficulty in culturing a diverse array of filamentous fungal species due to their (i) often unpredictable growth, (ii) unknown preferences for culturing conditions, and (iii) long incubation times compared with other microorganisms such as bacteria and yeasts. Given the complexity associated with concurrently culturing diverse fungal species, developing practical methods for preserving as many species as possible for future research is vital. The widely accepted best practice for preserving fungal tissue is the use of cryogenic biobanking at -165 C, allowing for the preservation and documentation of stable genetic lineages, thus enabling long-term diversity-centered research. Despite the extensive literature on fungal cryopreservation, substantial barriers remain for implementation of cryogenic biobanks in smaller mycological laboratories. In this work, we present practical considerations for the establishment of a fungal culture biobank, as well as provide evidence for the viability of 61 fungal genera in cryogenic storage. By providing a pragmatic methodology for cryogenically preserving and managing many filamentous fungi, we show that creating a biobank can be economical for independently owned and operated mycology laboratories, which can serve as a long-term resource for biodiversity, conservation, and strain maintenance.
摘要：
真菌占据重要的环境,文化,社会经济角色。然而,这个多元化王国的生物学研究落后于其他系统发育群体。部分原因是由于（i）通常无法预测的生长，因此在培养多种丝状真菌物种方面存在着臭名昭著的困难。(ii)对培养条件的偏好未知，和(iii)与其它微生物如细菌和酵母相比,培养时间长。考虑到与同时培养不同真菌物种相关的复杂性，开发实用的方法来保存尽可能多的物种，为未来的研究是至关重要的。广泛接受的保存真菌组织的最佳实践是在-165°C下使用低温生物库，允许保存和记录稳定的遗传谱系，从而实现以多样性为中心的长期研究。尽管有大量关于真菌冷冻保存的文献，在较小的真菌学实验室中，低温生物库的实施仍然存在很大的障碍。在这项工作中,我们提出了建立真菌培养生物库的实际考虑，以及为61个真菌属在低温储存中的生存能力提供证据。通过提供一种实用的方法来低温保存和管理许多丝状真菌，我们表明，创建生物库对于独立拥有和运营的真菌学实验室来说是经济的，它可以作为生物多样性的长期资源，养护,和应变维护。
