Abstract：
Whole genome sequencing (WGS) is becoming an important diagnostic tool for antimicrobial susceptibility testing of Mycobacterium tuberculosis complex (MTBC) isolates in many countries. WGS protocols usually start with the preparation of a DNA-library: the critical first step in the process. A DNA-library represents the genomic content of a DNA sample and consists of unique short DNA fragments. Although available DNA-library protocols come with manufacturer instructions, details of the entire process, including quality controls, instrument parameters, and run evaluations, often need to be developed and customized by each laboratory to implement WGS technology effectively. Here, we provide a detailed workflow for a DNA-library preparation based on an adapted Illumina protocol optimized for the reduction of reagent costs.
摘要：
在许多国家，全基因组测序（WGS）正在成为结核分枝杆菌复合体（MTBC）分离株抗菌药物敏感性测试的重要诊断工具。WGS方案通常从DNA文库的制备开始:该过程中关键的第一步。DNA文库代表DNA样品的基因组内容并且由独特的短DNA片段组成。尽管可用的DNA文库协议带有制造商说明，整个过程的细节，包括质量控制，仪器参数,运行评估，通常需要由每个实验室开发和定制，以有效地实施WGS技术。这里,我们提供了DNA文库制备的详细工作流程,该流程基于为降低试剂成本而优化的Illumina方案.
