Abstract：
Outbreaks are a risk to public health particularly when pathogenic, hypervirulent, and/or multidrug-resistant organisms (MDROs) are involved. In a hospital setting, vulnerable populations such as the immunosuppressed, intensive care patients, and neonates are most at risk. Rapid and accurate outbreak detection is essential to implement effective interventions in clinical areas to control and stop further transmission. Advances in the field of whole genome sequencing (WGS) have resulted in lowered costs, increased capacity, and improved reproducibility of results. WGS now has the potential to revolutionize the investigation and management of outbreaks replacing conventional genotyping and other discrimination systems. Here, we outline specific procedures and protocols to implement WGS into investigation of outbreaks in healthcare settings.
摘要：
疫情对公众健康构成风险，特别是在致病时，高毒力，和/或多重耐药生物体(MDRO)参与。在医院里，脆弱的人群，如免疫抑制者，重症监护病人,新生儿的风险最大。快速准确的疫情检测对于在临床领域实施有效的干预措施以控制和阻止进一步的传播至关重要。全基因组测序（WGS）领域的进步降低了成本，容量增加，并提高了结果的可重复性。WGS现在有可能彻底改变疫情的调查和管理，取代传统的基因分型和其他歧视系统。这里,我们概述了实施WGS调查医疗机构疫情暴发的具体程序和方案.
