Abstract：
Tuberculosis (TB) is the most common cause of death from an infectious disease. Although treatment has been available for more than 70 years, it still takes too long and many patients default risking relapse and the emergence of resistance. It is known that lipid-rich, phenotypically antibiotic-tolerant, bacteria are more resistant to antibiotics and may be responsible for relapse necessitating extended therapy. Using a microfluidic system that acoustically traps live mycobacteria, M. smegmatis, a model organism for M. tuberculosis we can perform optical analysis in the form of wavelength-modulated Raman spectroscopy (WMRS) on the trapped organisms. This system can allow observations of the mycobacteria for up to 8 h. By adding antibiotics, it is possible to study the effect of antibiotics in real-time by comparing the Raman fingerprints in comparison to the unstressed condition. This microfluidic platform may be used to study any microorganism and to dynamically monitor its response to many conditions including antibiotic stress, and changes in the growth media. This opens the possibility of understanding better the stimuli that trigger the lipid-rich downregulated and phenotypically antibiotic-resistant cell state.
摘要：
结核病（TB）是传染病死亡的最常见原因。虽然治疗已经有70多年了，它仍然需要太长时间，许多患者面临复发和耐药性出现的风险。众所周知，富含脂质，表型抗生素耐受性，细菌对抗生素的耐药性更强，可能是复发的原因，需要延长治疗。使用微流体系统在声学上捕获活的分枝杆菌，M.污名涂片，我们可以以波长调制拉曼光谱（WMRS）的形式对被困生物进行光学分析。该系统可以观察分枝杆菌长达8小时。通过添加抗生素，通过比较拉曼指纹与无应力条件相比，可以实时研究抗生素的作用。这种微流体平台可用于研究任何微生物，并动态监测其对许多条件的反应，包括抗生素应激。和增长媒介的变化。这打开了更好地理解触发富含脂质的下调和表型抗生素抗性细胞状态的刺激的可能性。
