Abstract：
Recent research has indicated that Panax notoginseng saponins (PNS) extracted from the radix of Panax notoginseng (Burkill) F. H. Chen exert antidepressant effects. This study aimed to assess the antidepressive effects of ginsenoside Rg1 and PNS in a depression model induced by chronic unpredictable mild stress (CUMS). Over a period of three weeks, rats were administered ginsenoside Rg1 at a dose of 30 mg/kg and PNS at dosages ranging from 100 to 200 mg/kg body weight per day. To assess how ginsenoside Rg1 and PNS influence depression-like behaviours in rats, various assessments were conducted, including coat state evaluation, forced swim test, and elevated plus maze test. The levels of cortisol and testosterone in serum samples were analysed using the liquid chromatography-electrospray ionisation tandem mass spectrometry (LC-ESI-MS/MS) method. LC-ESI-MS/MS method provides precise and accurate results. The lower limit of quantification values for cortisol and testosterone were determined as 100 and 2 pg/mL, respectively. Our data demonstrated that both ginsenoside Rg1 and PNS significantly reversed depression-like behaviour in rats by improving coat condition, reducing immobility time in the forced swim test, and increasing time spent in the open arms of the elevated plus maze test. Furthermore, ginsenoside Rg1 and PNS exhibited a regulatory effect on cortisol and testosterone levels in plasma. These findings suggest that ginsenoside Rg1 and PNS may be potential antidepressants in clinical treatment.
摘要：
最近的研究表明，从三七（Burkill）F.H.Chen中提取的三七皂苷（PNS）具有抗抑郁作用。本研究旨在评估人参皂苷Rg1和PNS在慢性不可预知轻度应激（CUMS）诱导的抑郁症模型中的抗抑郁作用。在三周的时间里，给大鼠施用剂量为30mg/kg的人参皂苷Rg1和剂量范围为每天100至200mg/kg体重的PNS。探讨人参皂苷Rg1和PNS对大鼠抑郁样行为的影响,进行了各种评估，包括涂层状态评估，强迫游泳测试,和高架加迷宫测试。使用液相色谱-电喷雾电离串联质谱（LC-ESI-MS/MS）方法分析血清样品中皮质醇和睾丸激素的水平。LC-ESI-MS/MS方法提供了精确和准确的结果。皮质醇和睾酮的定量值的下限被确定为100和2pg/mL，分别。我们的数据表明，人参皂苷Rg1和PNS均通过改善皮毛状况显着逆转大鼠的抑郁样行为，减少强迫游泳测试中的不动时间，并增加了在高架迷宫测试的张开双臂中花费的时间。此外，人参皂苷Rg1和PNS对血浆皮质醇和睾酮水平具有调节作用。提示人参皂苷Rg1和PNS在临床治疗中可能是潜在的抗抑郁药。
