UNASSIGNED: Morphological and molecular techniques were employed to identify the louse species. Molecular identification methods and gene sequencing were used to identify resistance-associated mutations in the voltage-gated sodium channel (VGSC) gene.
UNASSIGNED: The results revealed that six amino acid substitutions, including threonine-to-isoleucine (T917I), leucine-to-phenylalanine (L920F), isoleucine-to-phenylalanine (I927F), phenylalanine-to-alanine (F928A), valine-to-arginine (V929R), and arginine-to-leucine (R930L) mutations, were present in the VGSC gene of L. stenopsis lice from various regions of Iran. These findings suggest the potential for pyrethroid resistance development in this louse species, highlighting the importance of integrated pest management (IPM) strategies. Such strategies, which combine selective insecticides, regular grooming, and environmental sanitation, are crucial for effectively managing L. stenopsis infestations and preserving the efficacy of pyrethroids for pest control. Moreover, the emergence of novel kdr mutations underscores the need for ongoing research into the molecular mechanisms underlying these mutations. This research is vital for developing strategies to combat pyrethroid resistance and maintaining the efficacy of insecticides in controlling lice.
■采用形态学和分子技术来鉴定虱类。使用分子鉴定方法和基因测序来鉴定电压门控钠通道(VGSC)基因中的抗性相关突变。
■结果显示,六个氨基酸取代,包括苏氨酸至异亮氨酸(T917I),亮氨酸至苯丙氨酸(L920F),异亮氨酸至苯丙氨酸(I927F),苯丙氨酸-丙氨酸(F928A),缬氨酸至精氨酸(V929R),和精氨酸-亮氨酸(R930L)突变,存在于来自伊朗各个地区的L.stenopsis虱子的VGSC基因中。这些发现表明在这种虱子物种中产生拟除虫菊酯抗性的潜力,强调病虫害综合治理(IPM)战略的重要性。这种策略,结合了选择性杀虫剂,定期梳理,和环境卫生,对于有效管理狭窄的L.stenopsis感染和保持拟除虫菊酯控制害虫的功效至关重要。此外,新的kdr突变的出现强调了对这些突变背后的分子机制进行持续研究的必要性。这项研究对于制定对抗拟除虫菊酯抗性的策略和保持杀虫剂控制虱子的功效至关重要。