PDF(Pubmed)
Abstract：
UNASSIGNED: Dengue disease severity and progression are determined by the host immune response, with both pro- and anti-inflammatory cytokines are key mediators.
UNASSIGNED: To study pro- and anti-inflammatory cytokines across dengue severity and as a biomarker for predicting severe dengue infection.
UNASSIGNED: Hospital-based cross-sectional study was conducted on 125 dengue-positive subjects across the 5-60 years age group of either gender in 2022.
UNASSIGNED: Haematological parameters and blood samples were drawn to measure cytokines IL6, IL-10 and TNF alpha using the ELISA technique.
UNASSIGNED: One-way ANOVA and the Kruskal - Wallis test were used to compare the dependent variables across categories of the dengue spectrum. Receiver operating characteristic curve was drawn to calculate the predictability of the cytokines as a predictor of severe dengue. A P < 0.05 was considered significant.
UNASSIGNED: 34.4% of cases had severe dengue infection with 53.2% of severe cases reported in >40 years of age. Only IL-6 levels significantly increased (P < 0.01) across the spectrum of dengue infection across age groups >20 years with a consistent and significant fall in platelet levels (P < 0.01). The accuracy of IL-6 to predict severe dengue was 74.4% and platelet count was 16.2%.
UNASSIGNED: Only IL-6 cytokine levels were significantly increased across the spectrum of dengue infection observed in age >20 years and can significantly predict the probability of severe dengue by 74% (sensitivity 81.4%). A significant decrease in platelet values is consistent with the severity but is not a good predictor for severe dengue infection.
UNASSIGNED: To study pro- and anti-inflammatory cytokines across dengue severity and as a biomarker for predicting severe dengue infection.
UNASSIGNED: Hospital-based cross-sectional study was conducted on 125 dengue-positive subjects across the 5-60 years age group of either gender in 2022.
UNASSIGNED: Haematological parameters and blood samples were drawn to measure cytokines IL6, IL-10 and TNF alpha using the ELISA technique.
UNASSIGNED: One-way ANOVA and the Kruskal - Wallis test were used to compare the dependent variables across categories of the dengue spectrum. Receiver operating characteristic curve was drawn to calculate the predictability of the cytokines as a predictor of severe dengue. A P < 0.05 was considered significant.
UNASSIGNED: 34.4% of cases had severe dengue infection with 53.2% of severe cases reported in >40 years of age. Only IL-6 levels significantly increased (P < 0.01) across the spectrum of dengue infection across age groups >20 years with a consistent and significant fall in platelet levels (P < 0.01). The accuracy of IL-6 to predict severe dengue was 74.4% and platelet count was 16.2%.
UNASSIGNED: Only IL-6 cytokine levels were significantly increased across the spectrum of dengue infection observed in age >20 years and can significantly predict the probability of severe dengue by 74% (sensitivity 81.4%). A significant decrease in platelet values is consistent with the severity but is not a good predictor for severe dengue infection.
摘要：
■登革热疾病的严重程度和进展取决于宿主的免疫反应，促炎和抗炎细胞因子是关键介质。
■研究不同登革热严重程度的促炎和抗炎细胞因子，并作为预测严重登革热感染的生物标志物。
■2022年，对5-60岁年龄组中任一性别的125名登革热阳性受试者进行了基于医院的横断面研究。
■使用ELISA技术绘制血液学参数和血液样品以测量细胞因子IL6，IL-10和TNFα。
■单因素方差分析和Kruskal-Wallis检验用于比较登革热谱的不同类别的因变量。绘制受试者工作特征曲线以计算细胞因子作为严重登革热预测因子的可预测性。P<0.05被认为是显著的。
■34.4%的病例有严重登革热感染，53.2%的严重病例报告年龄>40岁。在20岁以上年龄组的登革热感染范围内，只有IL-6水平显着增加（P<0.01），血小板水平一致且显着下降（P<0.01）。IL-6预测严重登革热的准确性为74.4%，血小板计数为16.2%。
仅IL-6细胞因子水平在年龄>20岁的登革热感染谱中显著增加,并且可以显著预测严重登革热的概率为74%(灵敏度81.4%)。血小板值的显着降低与严重程度一致，但不是严重登革热感染的良好预测指标。
■研究不同登革热严重程度的促炎和抗炎细胞因子，并作为预测严重登革热感染的生物标志物。
■2022年，对5-60岁年龄组中任一性别的125名登革热阳性受试者进行了基于医院的横断面研究。
■使用ELISA技术绘制血液学参数和血液样品以测量细胞因子IL6，IL-10和TNFα。
■单因素方差分析和Kruskal-Wallis检验用于比较登革热谱的不同类别的因变量。绘制受试者工作特征曲线以计算细胞因子作为严重登革热预测因子的可预测性。P<0.05被认为是显著的。
■34.4%的病例有严重登革热感染，53.2%的严重病例报告年龄>40岁。在20岁以上年龄组的登革热感染范围内，只有IL-6水平显着增加（P<0.01），血小板水平一致且显着下降（P<0.01）。IL-6预测严重登革热的准确性为74.4%，血小板计数为16.2%。
仅IL-6细胞因子水平在年龄>20岁的登革热感染谱中显著增加,并且可以显著预测严重登革热的概率为74%(灵敏度81.4%)。血小板值的显着降低与严重程度一致，但不是严重登革热感染的良好预测指标。
