PDF(Pubmed)
Abstract：
Tyrosine-protein kinase SYK, encoded by the SYK gene, is a non-receptor type protein kinase which mediates immune signal transduction through immunoreceptors. Tyrosine-protein kinase SYK expression has been associated with the development of various inflammatory diseases, cancer and neurodegenerative conditions. The reproducibility of tyrosine-protein kinase SYK research would help elucidate the mechanism in which it causes neuroinflammation as well as its potential as a novel target to treat Alzheimer\'s disease. This would be facilitated with the availability of high-quality tyrosine-protein kinase SYK. In this study, we characterized thirteen tyrosine-protein kinase SYK commercial antibodies for Western Blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. We identified many high-performing antibodies and encourage readers to use this report as a guide to select the most appropriate antibody for their specific needs.
摘要：
酪氨酸蛋白激酶SYK,由SYK基因编码，是一种通过免疫受体介导免疫信号转导的非受体型蛋白激酶。酪氨酸蛋白激酶SYK的表达与多种炎症性疾病的发生发展有关,癌症和神经退行性疾病。酪氨酸蛋白激酶SYK研究的可重复性将有助于阐明其引起神经炎症的机制及其作为治疗阿尔茨海默病的新靶点的潜力。高质量酪氨酸蛋白激酶SYK的可获得性将促进这一点。在这项研究中,我们鉴定了13种酪氨酸蛋白激酶SYK商业抗体用于蛋白质印迹，免疫沉淀,和免疫荧光使用基于比较敲除细胞系和等基因亲本对照中的读数的标准化实验方案。我们确定了许多高性能抗体，并鼓励读者使用本报告作为指导，以选择最适合其特定需求的抗体。
