PDF(Pubmed)
Abstract：
UNASSIGNED: The evaluation of anti-apoptotic and chondroprotective properties of a single injection of PRP using immunohistochemistry (IHC).
UNASSIGNED: This was a placebo-controlled blinded experimental study. Ten healthy Dunkin Hartley guinea pigs were selected. One knee of each animal was injected with a single injection of PRP (Group A); the contralateral knee acted as a control and was injected with a single injection of normal saline (Group B). These groups were further divided into A3 and B3 based on the timeline of animal sacrifice (3 months) and A6 and B6 (6 months). The formalin-preserved articular cartilage blocks were subjected to IHC to stain Aggrecan, Caspase-3, and Collagen-2.
UNASSIGNED: The mean IHC score was significantly low for Caspase-3 (p-0.029) in intervention group (A3) in comparison to placebo control group (B3) pointing towards decreased apoptosis. The mean IHC values were significantly higher for Collagen II (p-0.011) for intervention group (A6) in contrast to control group (B6); values were also significantly low for Caspase-3 (p-0.029) in A6 as compared to B6. The mean Caspase-3 values were significantly higher in A6 as compared to A3 (p-0.029).
UNASSIGNED: The impact of a solitary injection of PRP on upregulation of anabolic pathways inside cartilage is relatively slower as compared to its effect on downregulation of apoptotic pathways. Even a single PRP injection holds the potential to change cartilage microenvironment, but the effects are not long lasting.
UNASSIGNED: This was a placebo-controlled blinded experimental study. Ten healthy Dunkin Hartley guinea pigs were selected. One knee of each animal was injected with a single injection of PRP (Group A); the contralateral knee acted as a control and was injected with a single injection of normal saline (Group B). These groups were further divided into A3 and B3 based on the timeline of animal sacrifice (3 months) and A6 and B6 (6 months). The formalin-preserved articular cartilage blocks were subjected to IHC to stain Aggrecan, Caspase-3, and Collagen-2.
UNASSIGNED: The mean IHC score was significantly low for Caspase-3 (p-0.029) in intervention group (A3) in comparison to placebo control group (B3) pointing towards decreased apoptosis. The mean IHC values were significantly higher for Collagen II (p-0.011) for intervention group (A6) in contrast to control group (B6); values were also significantly low for Caspase-3 (p-0.029) in A6 as compared to B6. The mean Caspase-3 values were significantly higher in A6 as compared to A3 (p-0.029).
UNASSIGNED: The impact of a solitary injection of PRP on upregulation of anabolic pathways inside cartilage is relatively slower as compared to its effect on downregulation of apoptotic pathways. Even a single PRP injection holds the potential to change cartilage microenvironment, but the effects are not long lasting.
摘要：
■使用免疫组织化学（IHC）评估单次注射PRP的抗凋亡和软骨保护特性。
■这是安慰剂对照的盲法实验研究。选择10只健康的DunkinHartley豚鼠。每只动物的一个膝盖注射一次PRP（A组）；对侧膝盖作为对照，并注射一次生理盐水（B组）。基于动物处死的时间线(3个月)和A6和B6(6个月),将这些组进一步分为A3和B3。对福尔马林保存的关节软骨块进行IHC染色Aggrecan，胱天蛋白酶-3和胶原蛋白-2。
与安慰剂对照组(B3)相比,干预组(A3)中的Caspase-3(p-0.029)的平均IHC评分显著低,表明细胞凋亡减少。与对照组(B6)相比,干预组(A6)的II型胶原(p-0.011)的平均IHC值显著较高;与B6相比,A6中的Caspase-3(p-0.029)的值也显著较低。A6中的平均Caspase-3值显著高于A3(p-0.029)。
■单独注射PRP对软骨内合成代谢途径上调的影响与其对凋亡途径下调的影响相比相对较慢。即使是一次PRP注射也有可能改变软骨微环境，但是效果并不持久。
■这是安慰剂对照的盲法实验研究。选择10只健康的DunkinHartley豚鼠。每只动物的一个膝盖注射一次PRP（A组）；对侧膝盖作为对照，并注射一次生理盐水（B组）。基于动物处死的时间线(3个月)和A6和B6(6个月),将这些组进一步分为A3和B3。对福尔马林保存的关节软骨块进行IHC染色Aggrecan，胱天蛋白酶-3和胶原蛋白-2。
与安慰剂对照组(B3)相比,干预组(A3)中的Caspase-3(p-0.029)的平均IHC评分显著低,表明细胞凋亡减少。与对照组(B6)相比,干预组(A6)的II型胶原(p-0.011)的平均IHC值显著较高;与B6相比,A6中的Caspase-3(p-0.029)的值也显著较低。A6中的平均Caspase-3值显著高于A3(p-0.029)。
■单独注射PRP对软骨内合成代谢途径上调的影响与其对凋亡途径下调的影响相比相对较慢。即使是一次PRP注射也有可能改变软骨微环境，但是效果并不持久。
