PDF(Pubmed)
Abstract：
Wool plays an irreplaceable role in the lives of livestock and the textile industry. The variety of hair quality and shape leads to the diversity of its functions and applications, and the finer wool has a higher economic value. In this study, 10 coarse and 10 fine ordos fine wool sheep skin samples were collected for RNA-seq, and coarse and fine skin/hair follicle RNA-seq datasets of other five animal breeds were obtained from NCBI. Weighted gene co-expression network analysis showed that the common genes were clustered into eight modules. Similar gene expression patterns in sheep and rabbits with the same wool types, different gene expression patterns in animal species with different hair types, and brown modules were significantly correlated with species and breeds. GO and KEGG enrichment analyses showed that, most genes in the brown module associated with hair follicle development. Hence, gene expression patterns in skin tissues may determine hair morphology in animal. The analysis of differentially expressed genes revealed that 32 highly expressed candidate genes associated with the wool fineness of Ordos fine wool sheep. Among them, KAZALD1 (grey module), MYOC (brown module), C1QTNF6 (brown module), FOS (tan module), ITGAM, MX2, MX1, and IFI6 genes have been reported to be involved in the regulation of the hair follicle cycle or hair loss. Additionally, 12 genes, including KAZALD1, MYOC, C1QTNF6, and FOS, are differentially expressed across various animal breeds and species. The above results suggest that different sheep breeds share a similar molecular regulatory basis of wool fineness. Finally, the study provides a theoretical reference for molecular breeding of sheep breeds as well as for the investigation of the origin and evolution of animal hair.
摘要：
羊毛在畜牧业和纺织业的生活中起着不可替代的作用。头发质量和形状的多样性导致其功能和应用的多样性，细羊毛具有较高的经济价值。在这项研究中,收集10个粗、10个细鄂尔多斯细毛羊皮样本进行RNA-seq,从NCBI获得了其他五个动物品种的粗糙和精细皮肤/毛囊RNA-seq数据集。加权基因共表达网络分析显示,共有基因被聚类为8个模块。具有相同羊毛类型的绵羊和兔子中相似的基因表达模式，具有不同毛发类型的动物物种的不同基因表达模式，棕色模块与品种和品种显著相关。GO和KEGG富集分析表明，棕色模块中的大多数基因与毛囊发育有关。因此，皮肤组织中的基因表达模式可能决定动物的毛发形态。对差异表达基因的分析表明，32个高表达的候选基因与鄂尔多斯细毛羊的羊毛细度有关。其中,KAZALD1（灰色模块），MYOC（棕色模块），C1QTNF6（棕色模块），FOS(tan模块),ITGAM,据报道，MX2，MX1和IFI6基因参与毛囊周期或脱发的调节。此外,12个基因,包括AZALD1，MYOC，C1QTNF6和FOS,在各种动物品种和物种中差异表达。以上结果表明，不同绵羊品种具有相似的羊毛细度分子调控基础。最后,该研究为绵羊品种的分子育种以及动物毛发的起源和进化研究提供了理论参考。
