PDF(Pubmed)
Abstract：
UNASSIGNED: Candida albicans is an opportunistic pathogen that occurs as harmless commensals in the intestine, urogenital tract, and skin. It has been influenced by a variety of host conditions and has now evolved as a resistant strain. The aim of this study was thus detect the fluconazole resistant C. albicans from the root caries specimens and to computationally evaluate the interactions of an opaque-phase ABC transporter protein with the Psidium guajava bio-active compounds.
UNASSIGNED: 20 carious scrapings were collected from patients with root caries and processed for the isolation of C. albicans and was screened for fluconazole resistance. Genomic DNA was extracted and molecular characterization of Cdrp1 and Cdrp2 was done by PCR amplification. P. guajava methanolic extract was checked for the antifungal efficacy against the resistant strain of C. albicans. Further in-silico docking involves retrieval of ABC transporter protein and ligand optimization, molinspiration assessment on drug likeness, docking simulations and visualizations.
UNASSIGNED: 65% of the samples showed the presence of C.albicans and 2 strains were fluconazole resistant. Crude methanolic extract of P. guajava was found to be promising against the fluconazole resistant strains of C. albicans. In-silico docking analysis showed that Myricetin was a promising candidate with a high docking score and other drug ligand interaction scores.
UNASSIGNED: The current study emphasizes that bioactive compounds from Psidium guajava to be a promising candidate for treating candidiasis in fluconazole resistant strains of C. albicans However, further in-vivo studies have to be implemented for the experimental validation of the same in improving the oral health and hygiene.
UNASSIGNED: 20 carious scrapings were collected from patients with root caries and processed for the isolation of C. albicans and was screened for fluconazole resistance. Genomic DNA was extracted and molecular characterization of Cdrp1 and Cdrp2 was done by PCR amplification. P. guajava methanolic extract was checked for the antifungal efficacy against the resistant strain of C. albicans. Further in-silico docking involves retrieval of ABC transporter protein and ligand optimization, molinspiration assessment on drug likeness, docking simulations and visualizations.
UNASSIGNED: 65% of the samples showed the presence of C.albicans and 2 strains were fluconazole resistant. Crude methanolic extract of P. guajava was found to be promising against the fluconazole resistant strains of C. albicans. In-silico docking analysis showed that Myricetin was a promising candidate with a high docking score and other drug ligand interaction scores.
UNASSIGNED: The current study emphasizes that bioactive compounds from Psidium guajava to be a promising candidate for treating candidiasis in fluconazole resistant strains of C. albicans However, further in-vivo studies have to be implemented for the experimental validation of the same in improving the oral health and hygiene.
摘要：
■白色念珠菌是一种机会性病原体，在肠道中无害共生，泌尿生殖道,和皮肤。它受到多种宿主条件的影响，现已发展为抗性菌株。因此，这项研究的目的是从根龋标本中检测氟康唑抗性白色念珠菌，并通过计算评估不透明相ABC转运蛋白与番石榴生物活性化合物的相互作用。
■从患有根龋的患者中收集20个龋齿刮片，并进行处理以分离白色念珠菌，并筛选氟康唑耐药性。提取基因组DNA并通过PCR扩增进行Cdrp1和Cdrp2的分子表征。针对针对白色念珠菌的抗性菌株的抗真菌功效,检查了P.guajava甲醇提取物。进一步的计算机对接涉及ABC转运蛋白的检索和配体优化，关于药物相似性的摩尔灵感评估，对接模拟和可视化。
■65％的样品显示白色念珠菌的存在，2株对氟康唑耐药。发现番石榴的粗甲醇提取物有希望对抗白色念珠菌的氟康唑抗性菌株。计算机对接分析表明，杨梅素具有较高的对接得分和其他药物配体相互作用得分。
■目前的研究强调，番石榴的生物活性化合物是治疗白色念珠菌氟康唑耐药菌株念珠菌病的有希望的候选药物。必须进行进一步的体内研究，以实验验证其在改善口腔健康和卫生方面的作用。
■从患有根龋的患者中收集20个龋齿刮片，并进行处理以分离白色念珠菌，并筛选氟康唑耐药性。提取基因组DNA并通过PCR扩增进行Cdrp1和Cdrp2的分子表征。针对针对白色念珠菌的抗性菌株的抗真菌功效,检查了P.guajava甲醇提取物。进一步的计算机对接涉及ABC转运蛋白的检索和配体优化，关于药物相似性的摩尔灵感评估，对接模拟和可视化。
■65％的样品显示白色念珠菌的存在，2株对氟康唑耐药。发现番石榴的粗甲醇提取物有希望对抗白色念珠菌的氟康唑抗性菌株。计算机对接分析表明，杨梅素具有较高的对接得分和其他药物配体相互作用得分。
■目前的研究强调，番石榴的生物活性化合物是治疗白色念珠菌氟康唑耐药菌株念珠菌病的有希望的候选药物。必须进行进一步的体内研究，以实验验证其在改善口腔健康和卫生方面的作用。
