PDF(Pubmed)
Abstract：
Plasmodesmata are transmembrane channels embedded within the cell wall that can facilitate the intercellular communication in plants. Plasmodesmata callose-binding (PDCB) protein that associates with the plasmodesmata contributes to cell wall extension. Given that the elongation of cotton fiber cells correlates with the dynamics of the cell wall, this protein can be related to the cotton fiber elongation. This study sought to identify PDCB family members within the Gossypium. hirsutum genome and to elucidate their expression profiles. A total of 45 distinct family members were observed through the identification and screening processes. The analysis of their physicochemical properties revealed the similarity in the amino acid composition and molecular weight across most members. The phylogenetic analysis facilitated the construction of an evolutionary tree, categorizing these members into five groups mainly distributed on 20 chromosomes. The fine mapping results facilitated a tissue-specific examination of group V, revealing that the expression level of GhPDCB9 peaked five days after flowering. The VIGS experiments resulted in a marked decrease in the gene expression level and a significant reduction in the mature fiber length, averaging a shortening of 1.43-4.77 mm. The results indicated that GhPDCB9 played a pivotal role in the cotton fiber development and served as a candidate for enhancing cotton yield.
摘要：
胞浆是嵌入细胞壁内的跨膜通道，可以促进植物细胞间的通讯。与纤丝结合的纤丝结合蛋白（PDCB）有助于细胞壁延伸。鉴于棉纤维细胞的伸长与细胞壁的动力学相关，这种蛋白质可以与棉纤维的伸长有关。这项研究试图鉴定棉属中的PDCB家族成员。hirsutum基因组并阐明其表达谱。通过鉴定和筛选过程共观察到45个不同的家族成员。对其理化性质的分析揭示了大多数成员的氨基酸组成和分子量的相似性。系统发育分析促进了进化树的构建，将这些成员分为五组，主要分布在20条染色体上。精细的映射结果促进了第V组的组织特异性检查，显示GhPDCB9的表达水平在开花后五天达到峰值。VIGS实验导致基因表达水平显著下降，成熟纤维长度显著减少，平均缩短1.43-4.77毫米。结果表明，GhPDCB9在棉花纤维发育中起着举足轻重的作用，是提高棉花产量的候选材料。
