PDF(Pubmed)
Abstract：
UNASSIGNED: Astaxanthin (ASX) is a lipid-soluble keto-carotenoid with several biological effects. These effects may benefit polycystic ovarian syndrome (PCOS) patients. Imbalanced apoptosis/anti-apoptosis signaling has been considered the major pathogenesis of PCOS. In a randomized clinical trial, we tested the impact of ASX on the apoptotic pathway in PCOS granulosa cells (GCs). The present study hypothesizes that ASX may improve apoptosis in PCOS patients.
UNASSIGNED: This trial recruited patients with confirmed PCOS. A total of 58 patients were randomly assigned to take ASX (12 mg) or placebo for 8 weeks. Aspirated follicular fluid (FF) and blood samples were taken from both groups to measure BAX and BCL2 protein expression. Following FF aspiration, GCs from both groups were obtained; Real-Time PCR and Western blotting were used to evaluate the apoptotic pathway\'s gene and protein expression levels in GCs.BAXBCL2.
UNASSIGNED: In GCs analysis, ASX reduced DR5 gene and protein expression after 8 weeks compared to placebo(p<0.05). Also, Caspase8 (p>0.05) and BAX (p<0.05) gene expression declined, although the difference was not statistically significant for Caspase8. Besides,ASX treatment contributed to an elevated BCL2 gene expression in GCs(p<0.05). In FF and serum analysis, a statistically significant increase was found in BCL2 concentration in the ASX group (p<0.05). Moreover, a reduction in BAX level was confirmed in both FF and serum of the ASX group; however, this change was not significant in the serum (p>0.05).
UNASSIGNED: It seems that ASX consumption among women with PCOS improved serum and FF levels of apoptotic factors and modulated genes and protein expression of the apoptosis pathway in GCs. Nevertheless, further investigations are needed to reveal the potential role of this compound in PCOS treatment.
UNASSIGNED: This trial recruited patients with confirmed PCOS. A total of 58 patients were randomly assigned to take ASX (12 mg) or placebo for 8 weeks. Aspirated follicular fluid (FF) and blood samples were taken from both groups to measure BAX and BCL2 protein expression. Following FF aspiration, GCs from both groups were obtained; Real-Time PCR and Western blotting were used to evaluate the apoptotic pathway\'s gene and protein expression levels in GCs.BAXBCL2.
UNASSIGNED: In GCs analysis, ASX reduced DR5 gene and protein expression after 8 weeks compared to placebo(p<0.05). Also, Caspase8 (p>0.05) and BAX (p<0.05) gene expression declined, although the difference was not statistically significant for Caspase8. Besides,ASX treatment contributed to an elevated BCL2 gene expression in GCs(p<0.05). In FF and serum analysis, a statistically significant increase was found in BCL2 concentration in the ASX group (p<0.05). Moreover, a reduction in BAX level was confirmed in both FF and serum of the ASX group; however, this change was not significant in the serum (p>0.05).
UNASSIGNED: It seems that ASX consumption among women with PCOS improved serum and FF levels of apoptotic factors and modulated genes and protein expression of the apoptosis pathway in GCs. Nevertheless, further investigations are needed to reveal the potential role of this compound in PCOS treatment.
摘要：
■虾青素（ASX）是一种具有多种生物学效应的脂溶性酮类胡萝卜素。这些效应可能有益于多囊卵巢综合征(PCOS)患者。不平衡的凋亡/抗凋亡信号被认为是PCOS的主要发病机制。在一项随机临床试验中，我们检测了ASX对PCOS颗粒细胞凋亡途径的影响.本研究假设ASX可以改善PCOS患者的细胞凋亡。
■本试验招募了PCOS确诊患者。共有58名患者被随机分配服用ASX（12mg）或安慰剂8周。从两组中抽取卵泡液（FF）和血液样本以测量BAX和BCL2蛋白的表达。FF抽吸后,获得两组的GC；使用实时PCR和Western印迹评估GC中凋亡途径的基因和蛋白质表达水平。BAXBCL2.
■在GCs分析中，与安慰剂相比,ASX在8周后降低DR5基因和蛋白质表达(p<0.05)。此外，Caspase8(p>0.05)和BAX(p<0.05)基因表达下降,尽管Caspase8的差异无统计学意义。此外,ASX处理导致GCs中BCL2基因表达升高(p<0.05)。在FF和血清分析中，ASX组的BCL2浓度有统计学意义的增加(p<0.05).此外，ASX组的FF和血清中BAX水平均得到证实；然而，这种变化在血清中并不显著(p>0.05)。
■似乎PCOS女性的ASX消费改善了血清和FF凋亡因子水平，调节了GCs凋亡途径的基因和蛋白表达。然而，需要进一步的研究来揭示该化合物在PCOS治疗中的潜在作用.
■本试验招募了PCOS确诊患者。共有58名患者被随机分配服用ASX（12mg）或安慰剂8周。从两组中抽取卵泡液（FF）和血液样本以测量BAX和BCL2蛋白的表达。FF抽吸后,获得两组的GC；使用实时PCR和Western印迹评估GC中凋亡途径的基因和蛋白质表达水平。BAXBCL2.
■在GCs分析中，与安慰剂相比,ASX在8周后降低DR5基因和蛋白质表达(p<0.05)。此外，Caspase8(p>0.05)和BAX(p<0.05)基因表达下降,尽管Caspase8的差异无统计学意义。此外,ASX处理导致GCs中BCL2基因表达升高(p<0.05)。在FF和血清分析中，ASX组的BCL2浓度有统计学意义的增加(p<0.05).此外，ASX组的FF和血清中BAX水平均得到证实；然而，这种变化在血清中并不显著(p>0.05)。
■似乎PCOS女性的ASX消费改善了血清和FF凋亡因子水平，调节了GCs凋亡途径的基因和蛋白表达。然而，需要进一步的研究来揭示该化合物在PCOS治疗中的潜在作用.
