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Abstract：
UNASSIGNED: This study aimed to investigate the role of receptor tyrosine kinase-like orphan receptor 2 (ROR2) in triple-negative breast cancer (TNBC).
UNASSIGNED: ROR2 expression in primary TNBC and metastatic TNBC tissues was analyzed by immunohistochemical staining and PCR. ROR2 expression in TNBC cell lines was detected by PCR and Western blot analysis. The migration, invasion and chemosensitivity of TNBC cells with overexpression or knockdown of ROR2 were examined.
UNASSIGNED: ROR2 expression was high in metastatic TNBC tissues. ROR2 knockdown suppressed the migration, invasion and chemoresistance of TNBC cells. ROR2 overexpression in MDA-MB-435 cells promoted the migration, invasion, and chemoresistance. Moreover, ROR2 knockdown in HC1599 and MDA-MB-435 adriamycin-resistant cells enhanced chemosensitivity to adriamycin. ROR2 could activate PI3K/AKT/mTOR signaling in TNBC cells.
UNASSIGNED: ROR2 is upregulated and promotes metastatic phenotypes of TNBC by activating PI3K/AKT/mTOR signaling.
UNASSIGNED: ROR2 expression in primary TNBC and metastatic TNBC tissues was analyzed by immunohistochemical staining and PCR. ROR2 expression in TNBC cell lines was detected by PCR and Western blot analysis. The migration, invasion and chemosensitivity of TNBC cells with overexpression or knockdown of ROR2 were examined.
UNASSIGNED: ROR2 expression was high in metastatic TNBC tissues. ROR2 knockdown suppressed the migration, invasion and chemoresistance of TNBC cells. ROR2 overexpression in MDA-MB-435 cells promoted the migration, invasion, and chemoresistance. Moreover, ROR2 knockdown in HC1599 and MDA-MB-435 adriamycin-resistant cells enhanced chemosensitivity to adriamycin. ROR2 could activate PI3K/AKT/mTOR signaling in TNBC cells.
UNASSIGNED: ROR2 is upregulated and promotes metastatic phenotypes of TNBC by activating PI3K/AKT/mTOR signaling.
摘要：
■本研究旨在探讨受体酪氨酸激酶样孤儿受体2（ROR2）在三阴性乳腺癌（TNBC）中的作用。
■通过免疫组织化学染色和PCR分析原发性TNBC和转移性TNBC组织中的ROR2表达。通过PCR和Western印迹分析检测TNBC细胞系中的ROR2表达。移民,检测ROR2过表达或敲低的TNBC细胞的侵袭和化学敏感性。
■ROR2在转移性TNBC组织中高表达。ROR2击倒抑制了迁移，TNBC细胞的侵袭和化学抗性。ROR2过表达促进MDA-MB-435细胞的迁移,入侵,和化学抗性。此外，HC1599和MDA-MB-435阿霉素耐药细胞中的ROR2敲低增强了对阿霉素的化学敏感性。ROR2可以激活TNBC细胞中的PI3K/AKT/mTOR信号。
■ROR2上调并通过激活PI3K/AKT/mTOR信号促进TNBC的转移表型。
■通过免疫组织化学染色和PCR分析原发性TNBC和转移性TNBC组织中的ROR2表达。通过PCR和Western印迹分析检测TNBC细胞系中的ROR2表达。移民,检测ROR2过表达或敲低的TNBC细胞的侵袭和化学敏感性。
■ROR2在转移性TNBC组织中高表达。ROR2击倒抑制了迁移，TNBC细胞的侵袭和化学抗性。ROR2过表达促进MDA-MB-435细胞的迁移,入侵,和化学抗性。此外，HC1599和MDA-MB-435阿霉素耐药细胞中的ROR2敲低增强了对阿霉素的化学敏感性。ROR2可以激活TNBC细胞中的PI3K/AKT/mTOR信号。
■ROR2上调并通过激活PI3K/AKT/mTOR信号促进TNBC的转移表型。
