PDF(Pubmed)
Abstract：
UNASSIGNED: Maize/soybean intercropping is a common cropping practice in Chinese agriculture, known to boost crop yield and enhance soil fertility. However, the role of below-ground interactions, particularly root exudates, in maintaining intercropping advantages in soybean/maize intercropping systems remains unclear.
UNASSIGNED: This study aimed to investigate the differences in root exudates between intercropping and monocropping systems through two pot experiments using metabolomics methods. Multiple omics analyses were conducted to explore correlations between differential metabolites and the community of Arbuscular Mycorrhizal Fungi (AMF), shedding light on the mechanisms underlying the dominance of intercropping from the perspective of root exudates-soil microorganism interactions.
UNASSIGNED: The study revealed that intercropping significantly increased the types and contents of root exudates, lowered soil pH, increased the availability of nutrients like available nitrogen (AN) and available phosphorus (AP), and enhanced AMF colonization, resulting in improving the community composition of AMF. Besides, root exudates in intercropping systems differed significantly from those in monocropping, with 41 and 39 differential metabolites identified in the root exudates of soybean/maize, predominantly amino acids and organic acids. The total amount of amino acids in the root exudates of soybean intercropping was 3.61 times higher than in monocropping. Additionally, the addition of root exudates significantly improved the growth of soybean/maize and AMF colonization, with the mycorrhizal colonization rate in intercropping increased by 105.99% and 111.18% compared to monocropping, respectively. The identified metabolic pathways associated with root exudates were closely linked to plant growth, soil fertility improvement, and the formation of AMF. Correlation analysis revealed a significant relationship (P < 0.05) between certain metabolites such as tartaric acid, oxalic acid, malic acid, aspartic acid, alanine, and the AMF community. Notably, the photosynthetic carbon fixation pathway involving aspartic acid showed a strong association with the function of Glomus_f_Glomerace, the dominant genus of AMF. A combined analysis of metabolomics and high throughput sequencing revealed that the root exudates of soybean/maize intercropping have direct or indirect connections with AMF and soil nutrients.
UNASSIGNED: This suggests that the increased root exudates of the soybean/maize intercropping system mediate an improvement in AMF community composition, thereby influencing soil fertility and maintaining the advantage of intercropping.
UNASSIGNED: This study aimed to investigate the differences in root exudates between intercropping and monocropping systems through two pot experiments using metabolomics methods. Multiple omics analyses were conducted to explore correlations between differential metabolites and the community of Arbuscular Mycorrhizal Fungi (AMF), shedding light on the mechanisms underlying the dominance of intercropping from the perspective of root exudates-soil microorganism interactions.
UNASSIGNED: The study revealed that intercropping significantly increased the types and contents of root exudates, lowered soil pH, increased the availability of nutrients like available nitrogen (AN) and available phosphorus (AP), and enhanced AMF colonization, resulting in improving the community composition of AMF. Besides, root exudates in intercropping systems differed significantly from those in monocropping, with 41 and 39 differential metabolites identified in the root exudates of soybean/maize, predominantly amino acids and organic acids. The total amount of amino acids in the root exudates of soybean intercropping was 3.61 times higher than in monocropping. Additionally, the addition of root exudates significantly improved the growth of soybean/maize and AMF colonization, with the mycorrhizal colonization rate in intercropping increased by 105.99% and 111.18% compared to monocropping, respectively. The identified metabolic pathways associated with root exudates were closely linked to plant growth, soil fertility improvement, and the formation of AMF. Correlation analysis revealed a significant relationship (P < 0.05) between certain metabolites such as tartaric acid, oxalic acid, malic acid, aspartic acid, alanine, and the AMF community. Notably, the photosynthetic carbon fixation pathway involving aspartic acid showed a strong association with the function of Glomus_f_Glomerace, the dominant genus of AMF. A combined analysis of metabolomics and high throughput sequencing revealed that the root exudates of soybean/maize intercropping have direct or indirect connections with AMF and soil nutrients.
UNASSIGNED: This suggests that the increased root exudates of the soybean/maize intercropping system mediate an improvement in AMF community composition, thereby influencing soil fertility and maintaining the advantage of intercropping.
摘要：
■玉米/大豆间作是中国农业的一种常见种植方式，众所周知，可以提高作物产量和提高土壤肥力。然而,地下相互作用的作用，尤其是根系分泌物，在保持大豆/玉米间作系统的间作优势方面仍不清楚。
■本研究旨在通过使用代谢组学方法的两个盆栽实验来调查间作和单作系统之间根系分泌物的差异。进行了多个组学分析，以探索差异代谢产物与丛枝菌根真菌（AMF）群落之间的相关性。从根系分泌物-土壤微生物相互作用的角度揭示间作优势的机制。
■研究表明，间作显著增加了根系分泌物的类型和含量，降低土壤pH值，增加了养分的可用性，如有效氮(AN)和有效磷(AP)，增强了AMF的定植,从而改善了AMF的群落组成。此外,间作系统中的根系分泌物与单作系统中的根系分泌物显着不同，在大豆/玉米的根系分泌物中鉴定出41和39种差异代谢物，主要是氨基酸和有机酸。大豆间作根系分泌物中氨基酸的总量是单作的3.61倍。此外,根系分泌物的添加显着改善了大豆/玉米的生长和AMF定植，与单作相比，间作中的菌根定植率分别提高了105.99%和111.18%，分别。确定的与根系分泌物相关的代谢途径与植物生长密切相关，土壤肥力的改善，以及AMF的形成。相关分析显示某些代谢产物如酒石酸,草酸,苹果酸,天冬氨酸,丙氨酸,AMF社区。值得注意的是,涉及天冬氨酸的光合碳固定途径与Glomus_f_Glomerace的功能密切相关，AMF的显性属。代谢组学和高通量测序的联合分析表明，大豆/玉米间作的根系分泌物与AMF和土壤养分有直接或间接的联系。
■这表明大豆/玉米间作系统的根系分泌物增加介导了AMF群落组成的改善，从而影响土壤肥力，保持间作优势。
■本研究旨在通过使用代谢组学方法的两个盆栽实验来调查间作和单作系统之间根系分泌物的差异。进行了多个组学分析，以探索差异代谢产物与丛枝菌根真菌（AMF）群落之间的相关性。从根系分泌物-土壤微生物相互作用的角度揭示间作优势的机制。
■研究表明，间作显著增加了根系分泌物的类型和含量，降低土壤pH值，增加了养分的可用性，如有效氮(AN)和有效磷(AP)，增强了AMF的定植,从而改善了AMF的群落组成。此外,间作系统中的根系分泌物与单作系统中的根系分泌物显着不同，在大豆/玉米的根系分泌物中鉴定出41和39种差异代谢物，主要是氨基酸和有机酸。大豆间作根系分泌物中氨基酸的总量是单作的3.61倍。此外,根系分泌物的添加显着改善了大豆/玉米的生长和AMF定植，与单作相比，间作中的菌根定植率分别提高了105.99%和111.18%，分别。确定的与根系分泌物相关的代谢途径与植物生长密切相关，土壤肥力的改善，以及AMF的形成。相关分析显示某些代谢产物如酒石酸,草酸,苹果酸,天冬氨酸,丙氨酸,AMF社区。值得注意的是,涉及天冬氨酸的光合碳固定途径与Glomus_f_Glomerace的功能密切相关，AMF的显性属。代谢组学和高通量测序的联合分析表明，大豆/玉米间作的根系分泌物与AMF和土壤养分有直接或间接的联系。
■这表明大豆/玉米间作系统的根系分泌物增加介导了AMF群落组成的改善，从而影响土壤肥力，保持间作优势。
