PDF(Pubmed)
Abstract：
The emergence of Salmonella enterica serovars that produce extended-spectrum beta-lactamase and exhibit multi-drug resistance (MDR) poses a substantial global threat, contributing to widespread foodborne illnesses and presenting an alarming issue for public health. This study specifically concentrated on the isolation and identification of ESBL-resistant genes (bla TEM, bla SHV, bla CTX-M1, bla CTX-M2, bla CTX-M9, MultiCase ACC, MultiCase MOX, MultiCase DHA, bla OXA) and the antibiogram profiling of Salmonella enterica serovars found in goat meat samples procured from retail outlets in Bangladesh. During the research in the Sylhet district of Bangladesh, researchers gathered a total of 210 samples of goat meat from 13 different Upazilas. Primarily, cultural and biochemical methods were used for isolation of bacteria from the selected samples. Salmonella enterica serovars Typhimurium and Enteritidis, along with three ESBL-resistant genes, were identified through polymerase chain reactions (PCRs). The disk diffusion test was used to determine antimicrobial susceptibilities. Out of 210 samples analysed, Salmonella spp. was detected in 18.10 % (38 out of 210), with S. Enteritidis and S. Typhimurium found in 9.05 % (19 out of 210) and 5.24 % (11 out of 210) of the samples, respectively. A total of 72.73 % (8/11) of S. Enteritidis and 100 % (19/19) of S. Typhimurium isolates were positive by Multidrug-resistant patterns. The positive outcomes were found of S. Typhimurium tested 63.16 % (12 out of 19) for the bla TEM gene and 21.05 % (4/19) for the bla SHV, gene. The study proposes that the retail goat meat market channel could be a prominent transmission way of ESBL-producing MDR Salmonella enterica serovars, representing a significant public health hazard.
摘要：
产生超广谱β-内酰胺酶并表现出多药耐药性（MDR）的肠道沙门氏菌血清变型的出现构成了重大的全球威胁，导致广泛的食源性疾病，并给公共卫生带来令人震惊的问题。本研究特别集中于ESBL抗性基因的分离和鉴定(blaTEM，BLASHV,blaCTX-M1,blaCTX-M2,blaCTX-M9,MultiCaseACC,MultiCaseMOX,MultiCaseDHA,blaOXA）和从孟加拉国零售店购买的山羊肉样品中发现的肠沙门氏菌血清型的抗菌谱分析。在孟加拉国Sylhet区的研究中，研究人员从13个不同的Upazilas收集了总共210个山羊肉样本。首先,使用培养和生化方法从选定的样品中分离细菌。伤寒沙门氏菌和肠炎，以及三个抗ESBL基因，通过聚合酶链反应(PCR)鉴定。圆盘扩散测试用于确定抗微生物敏感性。在分析的210个样本中，沙门氏菌属。检测到18.10%(210人中有38人)，在9.05%(210个中的19个)和5.24%(210个中的11个)的样本中发现肠炎沙门氏菌和鼠伤寒沙门氏菌，分别。共有72.73%(8/11)的肠炎沙门氏菌和100%(19/19)的鼠伤寒沙门氏菌呈多重耐药阳性。BlaTEM基因检测到63.16%(19个中的12个)的鼠伤寒沙门氏菌阳性结果，BlaSHV检测到21.05%(4/19)的阳性结果，基因。研究认为，零售山羊肉市场渠道可能是产ESBL多药沙门氏菌肠道血清型的突出传播途径，代表着重大的公共健康危害。
