PDF(Pubmed)
Abstract：
UNASSIGNED: Feline panleukopenia is a contagious viral disease caused by the feline panleukopenia virus (FPV). A closely related pathogen is canine parvovirus (CPV), and amino acid substitutions in this virus allow it to acquire a feline host range. In feline hosts, the disease induced by CPV manifests with similar symptoms to those caused by FPV or milder ones, leading to its underdiagnosis. The aim of this study was to determine the presence of CPV type 2 (CPV-2) in cats with clinical symptoms of panleukopenia and to assess the use of commercial CPV antigen tests for the clinical diagnosis of FPV.
UNASSIGNED: Samples from 59 cats from central Slovakia were included in the study. Rectal swabs were collected and clinically tested for parvovirus infection using a commercial antigen test. Antigen-positive samples were confirmed by PCR targeting the viral VP2 gene. The sequences of the PCR products were established with the Sanger method.
UNASSIGNED: Of 59 samples, 23 were revealed to be positive for parvovirus infection by both antigen and PCR test (38.9%). Analysis with the National Center for Biotechnology Information BLASTn application showed 99.78-100% pairwise identity with FPV. The mortality rate of parvovirus-infected cats included in this study was 8.69% (2/23).
UNASSIGNED: Although feline disease with CPV-2 was not confirmed, the CPV antigen test was able to detect FPV infection.
UNASSIGNED: Samples from 59 cats from central Slovakia were included in the study. Rectal swabs were collected and clinically tested for parvovirus infection using a commercial antigen test. Antigen-positive samples were confirmed by PCR targeting the viral VP2 gene. The sequences of the PCR products were established with the Sanger method.
UNASSIGNED: Of 59 samples, 23 were revealed to be positive for parvovirus infection by both antigen and PCR test (38.9%). Analysis with the National Center for Biotechnology Information BLASTn application showed 99.78-100% pairwise identity with FPV. The mortality rate of parvovirus-infected cats included in this study was 8.69% (2/23).
UNASSIGNED: Although feline disease with CPV-2 was not confirmed, the CPV antigen test was able to detect FPV infection.
摘要：
■猫泛白细胞减少症是由猫泛白细胞减少症病毒（FPV）引起的一种传染性病毒性疾病。一种密切相关的病原体是犬细小病毒（CPV），和该病毒中的氨基酸取代使其能够获得猫科动物的宿主范围。在猫科动物宿主中，由CPV引起的疾病表现出与FPV或较温和的症状相似的症状，导致其诊断不足。这项研究的目的是确定具有泛白细胞减少症临床症状的猫中CPV2型（CPV-2）的存在，并评估将商业CPV抗原测试用于FPV的临床诊断。
■来自斯洛伐克中部的59只猫的样品被包括在研究中。收集直肠拭子并使用商业抗原测试对细小病毒感染进行临床测试。通过靶向病毒VP2基因的PCR确认抗原阳性样品。用Sanger法建立PCR产物的序列。
■在59个样本中，通过抗原和PCR测试发现23例细小病毒感染呈阳性（38.9％）。国家生物技术信息中心BLASTn应用的分析显示与FPV成对同一性99.78-100%。本研究中纳入的细小病毒感染猫的死亡率为8.69%(2/23)。
■尽管未证实患有CPV-2的猫科动物疾病，CPV抗原检测能够检测FPV感染.
■来自斯洛伐克中部的59只猫的样品被包括在研究中。收集直肠拭子并使用商业抗原测试对细小病毒感染进行临床测试。通过靶向病毒VP2基因的PCR确认抗原阳性样品。用Sanger法建立PCR产物的序列。
■在59个样本中，通过抗原和PCR测试发现23例细小病毒感染呈阳性（38.9％）。国家生物技术信息中心BLASTn应用的分析显示与FPV成对同一性99.78-100%。本研究中纳入的细小病毒感染猫的死亡率为8.69%(2/23)。
■尽管未证实患有CPV-2的猫科动物疾病，CPV抗原检测能够检测FPV感染.
