PDF(Pubmed)
Abstract：
UNASSIGNED: Outbreaks of fowl adenovirus (FAdV) infection in chicken flocks in Poland threaten birds\' health and lives and are rising in frequency. The risk of these infections in immunocompromised poultry flocks with developed clinical symptoms was analysed through virus detection in broiler chicks and correlation of cases with the birds\' immune strength.
UNASSIGNED: Samples were analysed from four broiler farms with chicks from the same hatchery in Silesia, Poland where feeding regimes were different. A normal diet was provided to birds on the control farm; a normal diet and probiotic, prebiotic, vitamin and microelement supplementation was supplied on another farm; a normal diet and antibiotics on the third; and a normal diet and both forms of supplementation were given on the fourth farm. Amplification of the virus DNA in a PCR with hexon gene L1 loop hypervariable region 1-4 primers determined the molecular characteristics of isolates of adenovirus strains obtained from necropsy tissue samples. The amplicon sequences were analysed, the pair-wise distances were determined, the maximum likelihood estimate for the gamma parameter for site rates was produced, Tajima\'s D neutrality test was run and the relative synonymous codon usage and transition/transversion bias were calculated.
UNASSIGNED: Two species and two serotypes of fowl adenovirus - MW353018-FAdV-1/A-L-liver and MW353019-FAdV-5/B-I-intestine - were isolated in three-week-old broiler chicks on the control farm.
UNASSIGNED: Supplementation of broiler chicken flocks with probiotics, prebiotics, vitamins and microelements may have a significant beneficial effect on immunity and can prevent virus infection. The studies provided new information on the molecular characteristics of adenovirus strains isolated from chicks with a low level of immunity.
UNASSIGNED: Samples were analysed from four broiler farms with chicks from the same hatchery in Silesia, Poland where feeding regimes were different. A normal diet was provided to birds on the control farm; a normal diet and probiotic, prebiotic, vitamin and microelement supplementation was supplied on another farm; a normal diet and antibiotics on the third; and a normal diet and both forms of supplementation were given on the fourth farm. Amplification of the virus DNA in a PCR with hexon gene L1 loop hypervariable region 1-4 primers determined the molecular characteristics of isolates of adenovirus strains obtained from necropsy tissue samples. The amplicon sequences were analysed, the pair-wise distances were determined, the maximum likelihood estimate for the gamma parameter for site rates was produced, Tajima\'s D neutrality test was run and the relative synonymous codon usage and transition/transversion bias were calculated.
UNASSIGNED: Two species and two serotypes of fowl adenovirus - MW353018-FAdV-1/A-L-liver and MW353019-FAdV-5/B-I-intestine - were isolated in three-week-old broiler chicks on the control farm.
UNASSIGNED: Supplementation of broiler chicken flocks with probiotics, prebiotics, vitamins and microelements may have a significant beneficial effect on immunity and can prevent virus infection. The studies provided new information on the molecular characteristics of adenovirus strains isolated from chicks with a low level of immunity.
摘要：
■在波兰的鸡群中，家禽腺病毒（FAdV）感染的爆发威胁着鸟类的健康和生命，并且频率正在上升。通过对肉鸡的病毒检测以及病例与禽类免疫强度的相关性，分析了在具有发展的临床症状的免疫功能低下的家禽群中这些感染的风险。
■分析了来自西里西亚同一孵化场的四个肉鸡养殖场的样品，波兰的喂养制度不同。向对照农场的鸟类提供正常饮食；正常饮食和益生菌，益生元,维生素和微量元素补充剂在另一个农场提供;正常饮食和抗生素在第三个农场;和正常饮食和两种形式的补充在第四个农场。用六邻体基因L1环高变区1-4引物在PCR中扩增病毒DNA，确定了从尸检组织样品中获得的腺病毒株分离株的分子特征。分析扩增子序列，确定了成对距离，产生了站点速率的伽马参数的最大似然估计，进行Tajima的D中性测试，并计算相对同义密码子使用和转换/颠换偏倚。
■在对照农场的三周龄肉鸡小鸡中分离出两种种类和两种血清型的家禽腺病毒-MW353018-FAdV-1/A-L-肝和MW353019-FAdV-5/B-I-肠。
■用益生菌补充肉鸡群，益生元,维生素和微量元素可能对免疫有显著的有益作用，并可以预防病毒感染。这些研究为从免疫水平低的雏鸡中分离出的腺病毒株的分子特征提供了新的信息。
■分析了来自西里西亚同一孵化场的四个肉鸡养殖场的样品，波兰的喂养制度不同。向对照农场的鸟类提供正常饮食；正常饮食和益生菌，益生元,维生素和微量元素补充剂在另一个农场提供;正常饮食和抗生素在第三个农场;和正常饮食和两种形式的补充在第四个农场。用六邻体基因L1环高变区1-4引物在PCR中扩增病毒DNA，确定了从尸检组织样品中获得的腺病毒株分离株的分子特征。分析扩增子序列，确定了成对距离，产生了站点速率的伽马参数的最大似然估计，进行Tajima的D中性测试，并计算相对同义密码子使用和转换/颠换偏倚。
■在对照农场的三周龄肉鸡小鸡中分离出两种种类和两种血清型的家禽腺病毒-MW353018-FAdV-1/A-L-肝和MW353019-FAdV-5/B-I-肠。
■用益生菌补充肉鸡群，益生元,维生素和微量元素可能对免疫有显著的有益作用，并可以预防病毒感染。这些研究为从免疫水平低的雏鸡中分离出的腺病毒株的分子特征提供了新的信息。
